多药耐药铜绿假单胞菌接合性质粒、转座子、整合子遗传标记研究  

Investigation of Mobile Genetic Elements in Multidrug-resistant Pseudomonas aeruginosa Strains

作　　者：焦梅[1] 林宁[2] 孙海平[2] 

机构地区：[1]南京医科大学第二附属医院检验科,江苏南京210011 [2]南京医科大学附属淮安第一医院检验科,江苏淮安223300

出　　处：《中华医院感染学杂志》2010年第22期3455-3458,共4页Chinese Journal of Nosocomiology

摘　　要：目的调查多药耐药铜绿假单胞菌中接合性质粒、转座子、整合子等可移动遗传元件的存在情况。方法收集医院分离自痰液和伤口分泌物标本的铜绿假单胞菌共20株,采用聚合酶链反应(PCR)及序列分析的方法分析接合性质粒遗传标记(traA、traF、trbC)、转座子遗传标记(tnsA/Tn7、tnpA/Tn21、ISCR1、ISCR3/14、IS1133、IS26、ISpa7、ISkpa6、ISkpn7)、整合子遗传标记(intⅠ1、intⅠ2、intⅠ3),共15种可移动遗传元件。结果 20株铜绿假单胞菌共检出tnpA/Tn21、IS26、ISpa7、intⅠ1等4种基因,阳性株数分别为11株(55.0%)、2株(10.0%)、7株(35.0%)、11株(55.0%),其余11种基因未检出;只有8株(40.0%)未检出所进行检测的15种可移动遗传元件,但12株(60.0%)携带了>1种可移动遗传元件;阳性检出基因可分为6种构成模式,其中(tnpA/Tn21+ISpa7+intⅠ1)模式检出率最高,为6株(30.0%)。结论 20株铜绿假单胞菌未检出接合性质粒遗传标记,但检出的3种转座子和1种整合子遗传标记可能与铜绿假单胞菌呈多耐药的表型相关。OBJECTIVE To investigate the distribution of mobile genetic elements： plasmids, transposons, and integrons in multidrug-resistant Pseudornonas aeruginosa （P. aeruginosa） strains. METHODS A total of 20 strains of muhidrug-resistant P. aeruginosa isolated from sputum and wound secretion were collected, Then, 15 kinds of mobile genetic elements （traA, traF, trbC, tnsA /Tn7, tnpA/Tn21, ISCR1, ISCR3/14, ISl133, IS26, ISpaT,ISkpa6, ISkpnT, int I 1, int I 2, int I 3） were analyzed by PCR and verificated by DNA sequencing. RESULTS In 20 strains of P. aeruginosa, 11 strains（55.0%） ,2 strains（10.0%） ,7 strains（35.0%）and 11 strains （55.0%）were detected to carry tnpA/Tn21, IS26, ISpaT, int I 1, respectively. However, the rest 11 kinds of genes couldn＇t be detected out. Moreover, 8 strains（40. 0%） were not carring mobile genetic elements, but 12 strains（60.0%） were detected to carry at the least a kind of mobile genetic element. Furthermore, positive genes could be divided into 6 kinds of positive modes, among which the detection rates of the mode （tnpA/Tn21＋ISpa7 ＋intI 1） were the highest with 6 strains（30. 0%）. CONCLUSION Plasmids are not detected in 20 strains of multidrug-resistant P. aeruginosa, but S kinds of transposons and 1 kind of integron were detected,which might play a role in muhidrug resistant phenotype.

关 键 词：铜绿假单胞菌 质粒 转座子 整合子 可移动遗传元件 多药耐药 

分 类 号：R378.991[医药卫生—病原生物学]