实验鼠重症急性胰腺炎时奥曲肽对细菌易位影响的质粒标记研究  

Effect of octreotide on bacterial translocation in severe acute pancreatitis: a study by recombinant plasmid labeling

作　　者：陈建中[1] 戴植本[1] 

机构地区：[1]同济医科大学附属同济医院

出　　处：《中华普通外科杂志》1999年第2期97-99,共3页Chinese Journal of General Surgery

摘　　要：目的评价奥曲肽对重症急性胰腺炎（ｓｅｖｅｒｅａｃｕｔｅｐａｎｃｒｅａｔｉｔｉｓ，ＳＡＰ）及其细菌易位的治疗作用。方法已定殖含重组质粒的大肠杆菌大鼠８０只随机分为４组，每组２０只。在预防治疗组及其对照组，于诱导ＳＡＰ前先以４μｇ／ｋｇ静脉注射后继以６μｇ·ｋｇ－１·ｈｒ－１灌注１小时奥曲肽。在治疗组及其对照组，于诱导ＳＡＰ后０５小时先静脉注射４μｇ／ｋｇ，以后继续皮下注射８μｇ／ｋｇ，８小时一次共２４小时。２４小时后杀鼠取肠系膜淋巴结及腹水作培养，并用质粒ＤＮＡ分析和抗菌谱对细菌加以鉴定识别。结果在预防治疗组肠系膜淋巴结和腹水细菌易位发生率分别为５５％（１１／２０）和２０％（４／２０），而在治疗组则分别为４７４％（９／１９）和１５８％（３／１９），与各自对照组相比，都显著降低（Ｐ＜００５）。预防治疗组和治疗组病死率分别为０％（０／２０）和５％（１／２０），与其对照组相比，也显著降低（Ｐ＜００５）。Objective To evaluate the therapeutic effect of octreotide on severe acute pancreatitis(SAP) and bacterial translocation(BT).Methods 80 rats which were colonized a strain of E.coli bearing the recombinant plasmid were randomized into 4 groups with 20 rats in each group. In pretreatment group and control, octreotide was given at a rate of 6 μg·kg -1 ·hr -1 for 1 hour following intravenous injection at a dose of 4 μg/kg before induction of SAP; In treatment group and control, octreotide was administered subcutaneously at rate of 6 μg·kg -1 every the other 8 hours for 24 hours following intravenous injection at a dose of 4 μg/kg half an hour after induction of SAP. The rats were sacrificed 24 hours after the induction of pancreatitis.Mesenteric lymph node (MLN) and peritoneal fluid were collected for culture.Bacteria were identified by antibiogram and plasmid DNA analysis. Results The incidence rates in BT of MLN and peritoneal fluid in pretreatment group were 55%(11/20) and 20%(4/20),and those in treatment group were 47 4%(9/19) and 15 8%(3/19),compared with the control( P <0 05). The mortality of rats in pretreatment group and treatment group was 0%(0/20) and 5%(1/20),compared with their control( P <0 05) . Conclusions Octreotide has a therapeutic effect on BT in rats with SAP.

关 键 词：胰腺炎 细菌易位 奥曲肽 质粒标记 大鼠 

分 类 号：R657.510.2[医药卫生—外科学]