沙棘果水溶多糖JS_1的分离鉴定  

Purification and characterization of water soluble polysaccharide JS 1 from Hippophae rhamnoides

作　　者：王桂云[1] 梁忠岩[1] 张丽萍[1] 苗春艳[1] 

机构地区：[1]东北师范大学生命科学学院

出　　处：《中国药学杂志》1999年第4期229-231,共3页Chinese Pharmaceutical Journal

摘　　要：目的：从已提取过水溶性多糖后的沙棘果残渣中，进一步用稀碱液（０．１ｍｏｌ·Ｌ－１ＮａＯＨ溶液）提取多糖ＪＳ１，并研究其组成性质。方法：利用ＤＥＡＥＳｅｐｈａｄｅｘＡ２５柱层析法纯化沙棘果碱提水溶多糖，并采用玻璃纤维纸电泳，ＳｅｐｈａｒｏｓＣＬ４Ｂ柱层析比旋度等方法证明组分的均一性，同时用气相色谱、红外光谱等方法对其组成进行研究。结果：分离得到ＪＳ１水溶性多糖，经气相色谱分析得单糖组分为Ａｒａ，Ｘｙｌ，Ｇａｌ，Ｇｌｃ，摩尔比依次是１∶６∶１２∶４。结论：ＪＳ１为中性杂多糖。OBJECTIVE:To study the purification and characterization of water soluble polysaccharide JS 1 from Hippophae rahamnoides L.fruits that had been extracted with water.METHODS:JS 1 was purified by gel chromatography on DEAE Sep hadex A 25.its homogeneity was proved by gel chromatogrophy on Sepharose CL 4B glass fiber paper electrophoresis and specific rotator power.Its stral composition was studied by means of GC,IR,etc.RESULTS:JS 1 is a water solube polysaccharide.It is consisted of Ara,Xyl,Gal,Glc with the molarity rate of 1∶6∶12∶4 in turn by means of GC.CONCLUSION:JS 1 is a neutral heteropolysaccharide.

关 键 词：沙棘 沙棘多糖 分离 组成 JS1 

分 类 号：R282.71[医药卫生—中药学] R931.71[医药卫生—中医学]