4-氧和4-羟四甲基哌啶氮氧自由基及衍生物抗脂质过氧化作用研究  

The anti peroxidation of 4 oxy and 4 hydroxy nitroxides and its derivatives

作　　者：苏军华 李等松 吴勇杰 王绪明[1,2,3,4] 田暄 

机构地区：[1]兰州军区后勤部军事医学研究所 [2]兰州医学院 [3]兰州军区解放军医学高等专科学校 [4]兰州大学化学系国家重点实验室

出　　处：《中国药学杂志》1999年第4期243-246,共4页Chinese Pharmaceutical Journal

摘　　要：目的：对６种四甲基哌啶氮氧自由基及衍生物（４ＴＥＭＰＯ）抗脂质过氧化作用进行了实验研究，为其进一步研究及应用于临床作为抗自由基损伤药物提供依据。方法：用Ｆｅ２＋ＶｉｔＣ引发大鼠肝细胞膜、心肌细胞膜、肝线粒体的脂质过氧化，通过ＴＡＢ比色法测定丙二醛（ＭＤＡ）含量观测了４ＴＥＭＰＯ抗脂质过氧化作用。结果：ＯＴＭＰＯ（４氧２，２，６，６四甲基哌啶氮氧自由基）、ＨＴＭＰＯ（４羟２，２，６，６四甲基哌啶氮氧自由基）、ＨＴＭＰＯＨ（４羟２，２，６，６四甲基羟哌啶）、ＯＴＭＰＯＨ（４氧２，２，６，６四甲基羟哌啶）有较强的剂量依赖性抑制ＭＤＡ产生的作用，且ＯＴＭＰＯＨ，ＨＴＭＰＯＨ较ＯＴＭＰＯ，ＨＴＭＰＯ作用强，而ＯＴＭＰ（４氧２，２，６，６四甲基哌啶）、ＨＴＭＰ（４羟２，２，６，６四甲基哌啶）无效。结论：４ＴＥＭＰＯ有较明显的对抗脂质过氧化作用，作为一种临床抗自由基引起的脂质过氧化损伤的药物研究及开发利用，可能有广泛的前景。OBJECTIVE:The anti peroxidation of 4 TEMPO (4 oxy and 4 hydroxy nitroxides and its derivatives) were studied.METHODS:The anti peroxidation effect of 4 TEMPO in membranes form rat liver,heart and mitochondria were compared by the determination of malondialdehyde (MDA) using TBA colorimetry.RESULTS:OTMPO,HTMPO,OTMPOH and HTMPOH inhibited MDA generation with IC 50 6.27～22.32μmol·L -1 in different tissues.The effects of OTMPOH and HTMPOH were stronger than OTMPO and HTMPO.OTMP and HTMP had no effect.CONCLUSION:The results showed that 4 TEMPO were effective,The dosage and efficacy were obviously correlated.Therefore it may be necessary to study it further.

关 键 词：丙二醛 脂质过氧化 氧自由基 4-TEMPO 

分 类 号：R977.9[医药卫生—药品]