蛛网膜下腔移植骨髓间充质干细胞对大鼠慢性神经病理性痛的影响  

Effect of subarachnoid bone marrow mesenchymal stem cell transplantation on chronic neuropathic pain in rats

作　　者：王萍[1] 田学愎[1] 罗婷[1] 刘成[1] 方岩[1] 杨凯[1] 田玉科[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学研究室,武汉市430030

出　　处：《中华麻醉学杂志》2010年第7期829-832,共4页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金（30872441）;湖北省卫生厅科研基金（JX2807）

摘　　要：目的探讨蛛网膜下腔移植骨髓间充质干细胞（MSCs）对大鼠慢性神经病理性痛的影响。方法雌性SD大鼠140只，体重180～200g，采用坐骨神经分支选择损伤（SNI）法制备慢性神经病理性痛模型，术后2周，随机分为4组（n=35）：模型组（NP组）、MSCs组、PBS组和骨髓未贴壁单核细胞组（BNMCs组）。MSCs组、PBS组、BNMCs组蛛网膜下腔分别注射1×10 5／m的第3代MSCs悬液10ul、PBS10ul、1×10 5／ul的BNMCs悬液10ul。于术前、术后2周、移植后1、2、3、4、5周（T0-6）时测定机械痛阈。同时于T1～6时测定痛阈后随机选择5只大鼠取脊髓腰膨大组织，使用RT-PCR法测定脑源性神经营养因子（BDNF）mRNA表达。结果与T0时比较，T1时各组机械痛阈降低，T2-6时NP组、PBS组、BNMCs组机械痛阈降低（P〈0．05）。与，T1时比较，MSCs组T2-6时机械痛阈升高，T2～4时BDNFmRNA表达上调（P〈0．05），PBS组和BNMCs组其余时点各指标差异无统计学意义（P〉0．05）。与NP组比较，MSCs组机械痛阈升高，BDNFmRNA表达上调（P〈0．05），PBS组和BNMCs组各指标差异无统计学意义（P〉0．05）。结论蛛网膜下腔移植MSCs可减轻大鼠慢性神经病理性痛，其机制可能与上调脊髓BDNFmRNA表达有关。Objective To investigate the effect of transplantation of bone marrow mesenchymal stem cells （MSCs） in subarachnoid space on chronic neuropathic pain in rats. Methods One hundred and forty female SD rats weighing 180-200 g were used in this study. Chronic neuropathic pain （NP） was induced by ligation and separation of tibial and common fibular nerves （SNI）. Two weeks after the surgery the animals were randomly divided into 4 groups （n =35 each）： group I NP; group II NP＋ MSC （MSCs）; group III NP＋ phosphate buffer saline （PBS） and group IV NP ＋ bone marrow monocyte （BNMCs）. MSCs 10 ul, PBS 10 ul and BNMCs 10 ul were injected into subarachnoid space at 2 weeks after surgery in group II , III and IV respectively. Paw withdrawal threshold to von Frey filament stimulation （PWT） was measured before surgery （ T0, baseline）, at 2 weeks after surgery （T1） and 1, 2, 3, 4 and 5 weeks after subarachnoid injection （T2-6） . Five animals were killed at T1-6 in each group and their lumbar enlargements were removed for determination of BDNF mRNA expression. Results PWT was significantly decreased by SNI at T1 in all 4 groups, and at T2-6 in group I , III and IV as compared with the baseline at T0 （ P 〈 0.05） . Subarachnoid MSC transplantation significantly reduced mechanical hyperalgesia at T2-6 and up-regulated BDNF mRNA expression at T2-4 as compared with that at T1 （ P 〈 0.05）. There was no significant change in PWT and BDNF mRNA expression after subarachnoid PBS and BNMCs injection in group III and IV （P 〉 0.05）. Compared with group I , PWT was significantly increased and BDNF mRNA expression was up-regulated in group II （ P 〈 0.05 ）, but no significant change in PBS and BNMCs was found in group III （ P 〉 0.05） . Conclusion Up-regulation of BDNF mRNA expression in the spinal cord may be involved in the amelioration of chronic neuropathic pain by subarachnoid bone marrow mesenchymal stem cell transplantation.

关 键 词：间质干细胞移植 骨髓 蛛网膜下腔 神经痛 

分 类 号：R651.1[医药卫生—外科学]