水貂自咬症个体分子鉴定引物的开发与应用  

Development and application of primers for molecular identification of self-biting individuals among minks

作　　者：李玉梅[1] 丁雪梅[1] 孙博兴[1] 史旭东[1] 房恒通[1] 赵云[1] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062

出　　处：《兽类学报》2010年第4期456-459,共4页Acta Theriologica Sinica

基　　金：国家"863"计划资助项目(2007AA10Z150)

摘　　要：自咬症是危害笼养水貂（Mustla vison）的一种慢性疾病,其特征是阵发性神经高度兴奋、反复自咬身体后躯和尾部、对外界刺激敏感。常因外界刺激引起高度兴奋发作,干扰兽群繁殖。DNA samples obtained from healthy and self-biting Jinzhou Black minks were amplified by PCR with 100 random primers. A DNA fragment corresponding to self-biting cases was identified and marked as S356. The DNA fragment was purified and cloned into PMD18-T vector,subsequently,transformed into E. Coli JM109 and sequenced. We applied the SCAR label technology to detect the molecular marker S356 among the healthy and self-biting minks. Based on the sequence of S356,two pairs of SCAR primers,S3568001 and S3568002,were designed and used in PCR detection. The result showed that the target DNA fragment was amplified from 29 of 30 self-biting minks （ 96. 67% positive correspondence rate） with the SCAR labeling primers S3568001 and S3568002. However,there was no target fragment detected from healthy minks. The DNA fragment was denominated as DS356800,which could be used as a genetic marker to differentiate potential self-biting individuals from healthy minks,and could form a guideline for future anti-disease genetic breeding to eliminate the self-biting disease from mink populations.

关 键 词：水貂 自咬症 RAPD SCAR 分子标记 

分 类 号：Q751[生物学—分子生物学]