慢性复合应激诱发高血压动物模型的制备  

Preparation of chronic combined stimuli-induced hypertension animal model

作　　者：钟航海 冯英凯[2] 

机构地区：[1]重庆市九龙坡区第五人民医院内科,重庆400029 [2]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《现代医药卫生》2010年第23期3523-3525,共3页Journal of Modern Medicine & Health

摘　　要：目的:应用多重刺激手段,模拟上班白领族遭受的常见复合应激因素,制备慢性应激性高血压(SIH)大鼠模型,为进一步探讨SIH的发生、发展机制奠定基础。方法:健康成年Wistar大鼠30只随机分为传统模型组(n=10)、自建模型组(n=10)及正常对照组(n=10)。传统模型组接受足底电击结合工业噪声复合刺激,自建模型组应用不规则震荡、不良音乐、部分睡眠剥夺复合刺激法。正常对照组不接受应激刺激。建模时间为4周,在应激刺激0、7、14、21、28日(分别以D0、D1、D7、D14、D21、D28表示)测定各组收缩压及心率。第二十九日颈动脉插管取血4mL,分离血浆,酶联免疫吸附法(ELISA)法检测血浆血管紧张素Ⅱ(AngⅡ)及皮质醇水平,高效液相色谱(HPLC)法测定血浆儿茶酚胺含量。结果:传统模型组收缩压在应激刺激第七天较基础值上升约15mmHg,在第十四天达高峰(140.6±4.3)mmHg,而后略下降,但仍维持在高水平直至第二十八天;自建模型组SBP变化趋势与传统模型组类似,但上升时间及达高峰时间均晚1周;在观察期,正常对照组收缩压平稳。传统模型组及自建模型组心率在应激刺激1周后即开始增加,自第二周开始维持心率在较高水平,与正常对照组比较,差异有统计学意义(P<0.05)。传统模型组及自建模型组血浆血管紧张素Ⅱ、去甲肾上腺素、肾上腺素、皮质醇水平均显著高于正常对照组,差异均有统计学意义(P<0.05)。结论:应用不规则震荡、不良音乐、部分睡眠剥夺复合刺激法可成功诱发实验大鼠产生SIH,成功制备SIH大鼠模型。Objective：To develop the chronic stress induced hypertension（SIH） rat model by using multipe stimuli to simulate the common combined stressors from which the white-collar class suffers,so as to settle the basis for further investigation on occurrence and development of SIH.Methods：Total 30 healthy adult Wistar rats were randomly divided into traditional model group（n=10,receiving combined stimulation of electric foot shock and industrial noise）,self-made model group（n=10,receiving combined stimulation of irregular shake,infaust music and partial sleep deprivation） and normal control group（n=10,no stimuli given）.The modeling duration was 4 weeks.Systolic blood pressure（SBP） and heart rate（HR） were monitored at different time points（0,7,14,21,28 d after first stimuation,which were denoted as D0,D1,D7,D14,D21 and D28）.On 29 d,cervical arterial cannula was carried out to obtain the whole blood 4 mL;then the plasma was isolated.Enzyme linked immunosorbent assay（ELISA） was applied to determining plasma levels of angiotensin Ⅱ（AngⅡ） and cortisol,and high performance liquid chromatogram（HPLC） was adopted to measure the content of plasma catecholamine.Results：In traditional model group,SBP began to rise at D7 time pint,about 15 mmHg above the basic value,peaking at D14 time pint [the highest SBP was（140.6±4.3）mmHg],and declining afterwards,but still keeping a higher SBP level at D28 time point.The changing tendency of SBP in self-made model group was similar to that in traditional model group,notwithstanding the initial rising time and peaking time were one week later.In the viewing period,SBP kept stable in normal control group.Heart rate initiated to rise at D7,and from the second week on,HR kept at a higher level in traditional model group and self-made model group,significantly higher than that in normal control group（P0.05）.The plasma levels of AngⅡ,norepinephrine（NE）,epinephrin（E） and cortisol were markedly higher in traditional model group and sel

关 键 词：应激性高血压 动物模型 酶联免疫吸附试验 高效液相色谱 

分 类 号：R332[医药卫生—人体生理学]