梅花参糖胺聚糖的提取与分离纯化  

Isolation and purification of glycosaminoglycan from Thelenota ananas

作　　者：汪何雅[1] 刘义[1] 钱和[1] 

机构地区：[1]江南大学食品学院,无锡214122

出　　处：《食品科技》2010年第11期203-207,共5页Food Science and Technology

基　　金："十一五"国家科技支撑计划项目(2008BAD91B04-2)

摘　　要：研究梅花参糖胺聚糖的提取工艺和纯化方法,优化了乙醇和醋酸铅沉淀条件以及H2O2脱色条件,用DEAE-Cellulose和Sepharose CL-4B柱层析得到了梅花参糖胺聚糖,用琼脂糖凝胶电泳和高效凝胶渗透色谱法(HPGPC)验证了糖胺聚糖得纯度并得到了其分子量。结果表明,最佳乙醇沉淀浓度为45%(v/v),最佳醋酸钾沉淀浓度为1.2 mol/L,最佳H2O2脱色工艺参数为:H2O2浓度15%(v/v),反应温度60℃,反应时间4 h。纯化得到的梅花参糖胺聚糖(S5)为均一成分,其分子量为218289,它是由己糖醛酸、氨基己糖和岩藻糖组成的杂多糖,其糖链上有部分羟基发生硫酸酯化。Glycosaminoglycan（GAG） has many important physiological activities.In this paper,glycosaminog-lycan in body wall of Thelenota ananas was isolated by enzymic and alkaline hydrolysis,ethanol and lead acetate（KAc） precipitation,and purified by DEAE-Cellulose and Sepharose CL-4B column chromatography.The purity of purified GAG was studied by agarose gel electrophoresis and the molecular weight of it was studied by high performance gel permeation chromatography（HPGPC）.The results showed that the best GAG extraction conditions were： 45%（v/v）ethanol and 1.2 mol/L KAc precipitation.Then GAG was treated with H2O2 （15%,v/v） at 60 ℃ for 4 h for decoloration.The purified glycosaminoglycan of Thelenota ananas（fraction S5）was homogeneous and its molecular weight was 218289.It is a kind of heteropoly-saccharide composed of hexuronic acid,hexosamine,fucose and sulfate.

关 键 词：梅花参 糖胺聚糖 提取 纯化 

分 类 号：R284.2[医药卫生—中药学]