共焦扫描光学显微镜的高分辨率  

High resolution of the confocal scanning optical microscope

作　　者：高万荣[1] 陶纯堪[1] 聂守平[1] 刘明[1] 卞松龄[1] 

机构地区：[1]南京理工大学电光学院

出　　处：《光学技术》1999年第3期4-7,共4页Optical Technique

摘　　要：讨论了共焦扫描光学显微镜的高分辨率性质，指出共焦扫描显微镜由于采用点探测器，因而视场大大减小，信噪比大大提高，同时每幅图像逐点扫描形成，在光学系统信息能力不变的前提下，系统的空间域通带宽度增加和时域通带宽度减小。因而可成高分辨率的像，特别是其独特的深度分辨率特性使得可以实现光学断层扫描成像。Based on the concept of the invariance of the information capacity of an optical system, the property of the high resolution of the confocal scanning optical microscope is discussed. Because a point source and a point detector are used in a confocal microscope, the system is characterized by its very small field of view and high signal to noise ratio and has the decreased temporal bandwidth and increased spatial bandwidth. Therefore a high resolution image would be formed. The optical sectioning images obtained with the confocal fluorescent scanning microscope designed are given.

关 键 词：高分辨率 信息能力 荧光显微镜 共焦扫描 

分 类 号：TH742.65[机械工程—光学工程]