海茸多糖的分离纯化与结构表征  

Isolation,purification and structural character of polysaccharides from Durvillaea antarctica

作　　者：王培培[1] 于广利[1] 赵峡[1] 刘沙[1] 吴建东[1] 韩章润[1] 

机构地区：[1]中国海洋大学医药学院,山东省糖科学与糖工程重点实验室,山东青岛266003

出　　处：《中国海洋药物》2010年第5期1-5,共5页Chinese Journal of Marine Drugs

基　　金：国际合作(2007DFA30980);国家自然科学基金(30870506;31070724);国家海洋局专项(201005024)资助项目

摘　　要：目的从海茸(Durvillaea antarctica)中提取分离多糖并对其结构进行表征。方法采用冷水提取,然后用离子交换色谱柱分离纯化得到多糖HRC0和HRC1,用高效凝胶渗透色谱法(HPGPC)测定相对分子质量,用高效液相色谱法(HPLC)测定单糖组成,红外光谱(IR)和核磁共振波谱法(~1H-NMR,^(13)C-NMR)对其化学结构进行表征。结果 HRC0是一种相对分子质量为127.9 kD的线型β-1,3-葡聚糖;HRC1是一种相对分子质量为208.1 kD,且甘露糖醛酸含量高达83%的褐藻胶。结论海茸冷水提取物中主要是β-1,3-葡聚糖和高甘露糖醛酸含量的褐藻胶。Objective To extract and purify polysaccharides from Durvillaea antarctica and determine their structures. Methods The polysaccharides were extracted with cold water, then isolated and purified by ion exchange chromatography to get HRC0 and HRC1. The relative molecular mass was analyzed by high performance gel permeation chromatography （HPGPC）,monosaccharide composition was analyzed with high performance liquid chromatography （HPLC）. Their structural characters were confirmed by Fourier transform infrared spectroscopy （FTIR） and NMR（^1 H-NMR,^13C-NMR） techniques. Results HRC0 was a linear β-1,3-glucans with relative molecular mass of 127. 9 kD, and HRC1 was a alginate with high content of mannuronic acid, and its Mr was 208. 1 kD. Conclusion The cold-water extracts of Durvillaea antarctica were mainly composed by β -1,3-glucans and alginate with high content of mannuronic acid.

关 键 词：海茸 β-1 3-D-葡聚糖 聚甘露糖醛酸 结构 

分 类 号：R917[医药卫生—药物分析学] R284[医药卫生—药学]