刺松藻多糖对肝癌Hca-F荷瘤小鼠的抑瘤作用  

Inhibitory effect of the polysaccharides from Codium fragile on carcinoma Hca-F bearing mice

作　　者：王培胜[1] 刘宪丽[1] 刘东颖[1] 迟雅丽[1] 孙守旭[1] 邹向阳[1] 

机构地区：[1]大连医科大学生物技术系,辽宁大连116044

出　　处：《中国海洋药物》2010年第5期40-43,共4页Chinese Journal of Marine Drugs

基　　金：辽宁省教育厅高等学校科研计划项目(No.2009A199)

摘　　要：目的研究刺松藻多糖(CFPS)对小鼠Hca-F肝癌的生长抑制作用及机制。方法建立Hca-F实体型荷瘤小鼠模型,腹腔注射不同浓度的CFPS连续12d。称重法检测CFPS对肿瘤生长、脾脏和胸腺指数的影响;采用MTT法、ELISA法分析CFPS对荷瘤小鼠脾巨噬细胞吞噬活性、细胞因子分泌水平等免疫指标的影响。结果刺松藻多糖对小鼠实体瘤生长具有抑制作用,CFPS 200 mg·kg^(-1)·d^(-1)组与对照组相比有显著性差异(P<0.05);试验组小鼠脾指数和胸腺指数高于对照组。CFPS可增强脾巨噬细胞吞噬活性,100 mg·kg^(-1)·d^(-1)以上时可促进IL-2的产生。结论 CFPS对Hca-F肝癌小鼠肿瘤生长有抑制作用,可能与提高荷瘤小鼠细胞和分子免疫活性有关。Objective To investigate the antitumor activity of Codiurn fragile polysaccharides（CFPS）in Hca-F tumor-bearing mice and its mechanism. Methods Inhibition rate and immune organisc index of CFPS on Hca-F tumor-bearing mice in vivo. The phagocytic activity of macrophages was assessed by MTT assays, and the relative contents of IL-2 and TNF-α were detected by ELISA method. Results CFPS could obviously inhibit Hca-F carcinoma growth at dose of 200mg · kg^-1 for 12 days, the inhibition rate was 31.93%（P〈0.05） . The indices of spleen and thymus in test group （CFPS 100 mg · kg^-1） were higher than that in control. And the phagocytosis activity of spleenocyte and production of IL-2 were promoted by CFPS in test groups. Conclusion CFPS might inhibit the growth of Hca-F tumor cells through improving the immune function of immunocytes and cytokines in mice.

关 键 词：刺松藻 多糖 抗肿瘤 免疫 

分 类 号：R965[医药卫生—药理学]