胶州湾海域表层沉积物细菌多样性  

Diversity of bacterial community in sediments of Jiaozhou Bay

作　　者：刘欣[1,2] 肖天[1] 张文燕[1,3] 董逸[1,2] 岳海东[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049 [3]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋科学》2010年第10期1-6,27,共7页Marine Sciences

基　　金：国家自然科学基金项目(30770234;30910103914);山东省科技发展计划项目(2006GG2205023)

摘　　要：采用16S rDNA文库结合PCR-RFLP分析的手段,对胶州湾4个站位沉积物中的细菌多样性和群落特征研究分析。结果表明,沉积物中细菌种类丰富,最多包含分布于14个已知门类的细菌,和一些未被认知的序列;各站位沉积物中优势菌群均为变形菌门和酸杆菌门,其中γ-和δ-变形菌纲为变形菌门中的绝对优势类群,在4个文库序列中平均占42%和16.75%;此外,拟杆菌门、浮霉菌门和放线菌门的种类也较为大量存在。各细菌种群有较明显空间分布差异,可能与不同区域胶州湾环境条件相关。The 16S rDNA clone library technique and PCR-RFLP analysis were applied to give a comprehensive view of the diversities and compositions of sediment bacterial communities at four sampling stations of Jiaozhou Bay.The 16S rDNA clone library analysis indicated that the sediments contained high diversities of bacteria and distributed in 15 known phyla in maximum,and some sequences were affiliated to uncultured bacteria.Proteobac-teria and Acidobacteria were the dominant groups in the sediments.γ-Proteobacteria and δ-Proteobacteria,aver-agely accounting for 42% and 16.75% of the four libraries,respectively,were the most dominant classes in Proteo-bacteria.Members of Bacteroidetes,Planctomycetes and Actinobacteria were also comparatively dominant groups found in Jiaozhou Bay sediments.Bacterial community diversities differed in space,probably resulting from the special habitats of different microbial groups and also the different geographical locations in Jiaozhou Bay.

关 键 词：沉积物 细菌群落 16S rDNA多样性 胶州湾 

分 类 号：P736.21[天文地球—海洋地质]