海蜇养殖群体及自然捕获群体ITS序列遗传分析  

Genetic diversity of ITS sequences in farmed and natural Rhopilema esculentum populations

作　　者：孙国华[1,2] 刘相全[1] 杨建敏[1] 张锡佳[1] 刘爱英[1] 谭福奕 

机构地区：[1]山东省海洋水产研究所,山东烟台264006 [2]山东好当家海洋发展股份有限公司,山东威海264305

出　　处：《海洋科学》2010年第10期90-95,共6页Marine Sciences

基　　金：国家海洋局项目(海科字[2006]16号)

摘　　要：为调查海蜇（Rhopilema esculentum）自然海区群体和养殖群体遗传多样性,对烟台莱州湾和江苏海州湾自然海区捕获群体及威海养殖群体24个个体的ITS序列进行了PCR扩增和序列分析,获得DNA片段长度在1 080-1 096 bp之间,包括完整的ITS1,5.8S和完整ITS2序列。结果表明,在所测碱基序列中共发现26个变异位点,4处多碱基插入位置,29个插入缺失位点。群体内平均核苷酸差异数K和平均核苷酸多样性指数Pi为2.179-2.750和0.002 02-0.002 54,群体间平均核苷酸差异数K值波动在2.797-3.031之间,遗传距离在0.002 30-0.002 81之间,遗传分化指数（Fst）值在0.032 50-0.730 8之间,群体内及群体间遗传多样性指标数值比较相近,群体间的遗传距离比较小,群体遗传结构相似,群体间没有明显的遗传分化。For the purpose of investigating genetic diverstiy in wild and cultured populitions of Rhopilema escu-lentum,ITS regions of 24 individuals within Yantai and Jiangsu wild populitions and Weihai cultured population were amplified and analyzed.The whole sequences were between 1 080-1 096 bp,including ITS1-5.8S-ITS2.The result showed that 26 variable nucleotide positions were detected and four nucleotide sites had 29 insertion-deletion positions.Indexes of Pi and K among three populations were 2.179-2.750 and 0.002 02-0.002 54,respectively.In-dexes of K,genetic distance and fixation indices（Fst） between populations were 2.797-3.031,0.002 30-0.002 81 and 0.032 50-0.730 8,respectively.The indexs of genetic diversity among and between populations were closed and genetic distances between populations were small,which indicated that the genetic structure of populations were smilar and there were no distinct genetic differentiation between populations.

关 键 词：海蜇(Rhopilema esculentum) 转录间隔区(ITS) 遗传多样性 遗传结构 

分 类 号：Q347[生物学—遗传学]