一株以葡萄糖为底物产2,3-丁二醇微生物菌株的筛选及鉴定  

Screening and Identification of a Strain Producing 2,3-Butanediol from Glucose as Substrate

作　　者：林清 张显[1] 杨套伟[1] 徐美娟[1] 张术聪 夏海锋[1] 饶志明[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室江南大学生物工程学院,江苏无锡214122

出　　处：《微生物学通报》2010年第11期1581-1587,共7页Microbiology China

基　　金：国家863计划项目(No.2007AA02Z207);国家自然科学基金项目(No.30970056);霍英东青年基金项目(No.121020);中央高校基本科研业务费专项资金项目(No.JUSRP31001)

摘　　要：从自然界中筛选出一批以葡萄糖为底物发酵产2,3-丁二醇的菌株,经初步发酵测定发酵液中2,3-丁二醇含量,其中菌株6-7的2,3-丁二醇产量最高达49.6g/L。对其进行常规生理生化鉴定实验,并结合16SrDNA序列分析,比对结果表明,菌株6-7与Bacillus subtilis strain BIHB332相似性达99%。在细菌分类学上属于枯草芽孢杆菌属,将其命名为Bacillussubtilis6-7。其特点是属于环境友好和食品安全型菌株,因此,利用Bacillus subtilis6-7生产2,3-丁二醇具有良好的工业应用价值。Most strains with capability of producing 2,3-butanediol（2,3-BDO） from glucose were isolated from different natural environment samples.The Strain 6-7 with high capability of producing 2,3-BDO was screened from the isolated strains and the production of 2,3-BDO reached 49.6 g/L.In addition to general morphological and biochemical characteristics,the strain 6-7 was identified by 16S rDNA sequence and systematic analysis.The results showed that 16S rDNA sequence of strain 6-7 had similarity of 99% with Bacillus subtilis strain BIHB332,suggesting that the strain 6-7 is one of Bacillus subtilis species.It is an environmentally friendly strain and Bacillus subtilis is generally regarded as safe microorganism used for industrial production.This strain showed the potential application in the future.

关 键 词：2 3-丁二醇 分离筛选 鉴定 枯草芽孢杆菌 

分 类 号：TQ223.162[化学工程—有机化工]