标准菌株CMCC(F)98003的多相复核鉴定  

Polyphasic Identification Approaches for Reference Strain CMCC(F)98003

作　　者：姚粟[1] 李辉[1] 程池[1] 

机构地区：[1]中国食品发酵工业研究院中国工业微生物菌种保藏管理中心,北京100027

出　　处：《微生物学通报》2010年第11期1600-1605,共6页Microbiology China

基　　金：国家科技支撑计划项目(No.2007BAK36B06)

摘　　要：采用形态学观察、Biolog全自动微生物鉴定系统、ITSrDNA序列分析、ITS区特殊位点碱基分析、β-微管蛋白基因序列分析方法,对《中国药典》(CHP)中指定使用的培养基质控参考菌株CMCC(F)98003进行了多相复核鉴定。结果表明:CMCC(F)98003为黑曲霉,与CHP收载的科学名称是一致的。鉴于黑曲霉与巴西曲霉虽然较为接近,但已成为两个不同的种,其形态学、系统发育学和代谢产物等特性均存在一定差异,《中国药典》是否需要更换新的丝状真菌代表菌株,以保持与国际上其他药典的一致性值得研究。To identify reference strain CMCC（F）98003,which cited in Chinese Pharmacopoeia（CHP）,polyphasic approaches including morphological features,Biolog identification system,ITS rDNA sequence,specific positions based on the ITS region and β-tubulin gene sequences analysis were used.Results indicated that CMCC（F）98003 was identified as Aspergillus niger and consistent with the scientific name in CHP.Although A.brasiliensis is close to A.niger,they have been classified as two different species and their morphology,phylogenesis and metabolic profilies are distinct.To maintain the consistency of international Pharmacopoeia,whether CHP replaces the new representative of filamentous fungi strain is worth studying.

关 键 词：CMCC(F)98003 黑曲霉 巴西曲霉 多相鉴定 

分 类 号：Q93[生物学—微生物学]