应用基因芯片分析短日低温条件下新型粳稻光温敏核质互作不育系育性相关基因  

Analysis on Sterility-Related Genes in a New Type of Japonica Photo(thermo)-Sensitive Male Sterile Line under Short Daylength and Low Temperature Conditions by Microarray

作　　者：杜士云[1] 阳菁[2] 王守海[1] 王德正[1] 吴爽[1] 罗彦长[1] 李阳生[2] 

机构地区：[1]安徽省农业科学院水稻研究所,安徽合肥230031 [2]武汉大学生命科学学院,湖北武汉430070

出　　处：《中国水稻科学》2010年第6期559-566,共8页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30771379);国家863计划资助项目(2009AA101101)

摘　　要：为了解具有三系和两系两套不育机制的新型粳稻光温敏核质互作不育系2310SA分子水平上的育性机理,同时也试图对水稻雄性不育现象做进一步探讨,利用水稻基因芯片,以2310SA及其保持系两系光温敏不育系2310S为材料,在安徽合肥秋季自然短日低温条件下,比较了稳定不育的2310SA和恢复可育的2310S(对照)减数分裂期、单核期、二核期和三核期等4个穗发育时期花药基因表达谱的变化差异。减数分裂期(上调表达基因1938个,下调表达基因1635个)和三核期(上调表达基因2220个,下调表达基因2656个)差异表达基因数目多于单核期(上调表达基因752个,下调表达基因693个)和二核期(上调表达基因1025个,下调表达基因886个),三核期下调表达基因比上调表达基因多,其他3期上调表达基因比下调表达基因多。所有差异表达基因聚类显示,单核期和二核期差异表达基因聚为一类,减数分裂期和三核期差异表达基因独立成为第2类和第3类。筛选出147个在4个穗发育时期都差异表达的基因。这些共有差异表达基因也只有在三核期下调表达基因比上调表达基因多。这些共有差异表达基因,参与了生物合成、刺激反应、光合作用、信号传导、大分子的新陈代谢、运输、转录调节、碳水化合物的代谢、花的发育和细胞死亡等11类生物学过程。所得共有差异表达基因中存在与脂类代谢有关的细胞色素P450家族蛋白和β-环羟化酶,与热激反应有关的热激转录因子、热激蛋白DnaJ家族蛋白及热激蛋白70等相关基因。这些基因与细胞死亡有直接或间接的关系,它们的异常表达可能与不育花粉的形成有关。A new type of japonica photo（thermo）-sensitive cytoplasmic male sterile line 2310SA was bred.This line contains photo（thermo）-sensitive genic male sterile（PTGMS）genes in the two-line system hybrid rice and cytoplasmic male sterile（CMS）genes in the three-line system hybrid rice,which has a great application potential in hybrid rice breeding for its stable sterility.To understand the underlying genetic mechanism and to explore male sterility of rice,the selected 2310SA line and its maintainer line of the two-line system,PTGMS line 2310S,were grown in Hefei,China.The anthers were collected at four panicle developmental stages（meiosis,mononucleate,binucleate and mature stages）and the isolated RNA were used for microarray analysis.Results showed that differentially expressed genes（DEGs）at meiosis stage（1938genes up-regulated,1635genes down-regulated）and mature stage（2220genes up-regulated,2656genes down-regulated）were more than those at mononucleate stage（752genes up-regulated,693genes down-regulated）and binucleate stage（1025genes up-regulated,886 genes down-regulated）.The number of down-regulated genes was higher than that of up-regulated genes at mature stage,whereas at other stages the number of up-regulated genes was more than that of down-regulated genes.Moreover,hierarchical cluster analysis of all DEGs suggested that the expression of the DEGs at mononucleate and binucleate stages belonged to the same cluster,and the expression of the DEGs at meiosis and mature stages to another two clusters.Among DEGs,147genes were found to be differentially expressed at all the stages when comparing 2310SA line with the control line 2310S.For the 147 common DEGs,the down-regulated genes were more than the up-regulated ones at mature stage but not at other three stages.Additionally,these common DEGs were involved in many biological processes,which were classified into 11functional categories,including biosynthesis,response to stimulus,photosynthesis,macromolecule metabolic process,signal

关 键 词：基因芯片 粳稻 不育性 基因 光温敏核质互作不育系 光温敏不育系 

分 类 号：S511.22[农业科学—作物学]