水稻基因组DNA简易制备方法  

A Simple Method for Rapid Preparation of Rice Genomic DNA

作　　者：孙川[1,2] 陈刚[1,2] 饶玉春[1] 张光恒[1] 高振宇[1] 刘坚[1] 鞠培娜[1] 胡江[1] 郭龙彪[1] 钱前[1] 曾大力[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006 [2]扬州大学农学院,江苏扬州225009

出　　处：《中国水稻科学》2010年第6期677-680,共4页Chinese Journal of Rice Science

基　　金：国家转基因生物新品种培育重大科技专项资助项目(2008ZX08009-003);国家自然科学基金资助项目(30771160);浙江省自然科学基金杰出青年团队项目(R3090023)

摘　　要：介绍了一种用于PCR的水稻基因组DNA的快速制备方法。该方法只需将少量(1～50mg)样品、适量提取液(500μL)和一粒钢珠放入2mL离心管,在细胞破碎仪中粉碎2min,经离心后可直接取少量(约5μL)上清液作为PCR的模板DNA,也可进一步根据需要实现高质量DNA的提取。该方法具有成本低、操作快速简便等优点,一个人可以在10min内完成96份样品DNA的简易制备,特别适合高通量的分子检测。A simple method for preparation of rice genomic DNA was developed.A small amount（1-50mg）of leaf tissue of rice seedling,500μL of extraction buffer,and one steel bead were put into a 2-mL microcentrifuge tube.After vigorously mashing for 2min,5μL of the supernatant was directly applied to PCR amplification.Otherwise,the supernatant was precipitated with 2volumes of ethanol to obtain high quality genomic DNA.This method is simple,rapid,low cost,and reliable for PCR analysis.One person can manipulate as many as 96samples for PCR in 10minutes.It is especially suitable for genotyping of large number of samples.

关 键 词：DNA提取 高通量PCR扩增 样品制备 方法 水稻 

分 类 号：S511[农业科学—作物学]