应用新型调制荧光成像系统(Imaging-PAM)原位评估底泥毒性的方法  

AN IN-SITU ASSESSMENT OF SEDIMENT TOXICITY WITH THE APPLICATION OF IMAGING-PAM

作　　者：虞龙[1] 苗秀珍[1] 于洋[2] 张民[2] 阳振[2] 叶琳琳[2] 孔繁翔[2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,南京210008

出　　处：《环境化学》2010年第6期1116-1120,共5页Environmental Chemistry

基　　金：国家自然科学基金项目(20807043);中国科学院南京地理与湖泊研究所所长基金(07SL021001)资助

摘　　要：应用新型调制叶绿素荧光成像系统Imaging-PAM,通过最大叶绿素荧光量子产量(Fv/Fm)、实际荧光量子产量(YⅡ)指标的测定原位评估了友谊河底泥对斜生栅藻(Scenedesmus obliqnus)的毒性效应.数据显示,在友谊河底泥悬浮液中,栅藻的Fv/Fm和YⅡ值在12h前快速上升,12h后呈现出时间-效应型曲线,72h时Fv/Fm和YⅡ分别下降至对照的44%和35%,Fv/Fm和YⅡ的半抑制时间分别为57h和45h.同时,通过对友谊河底泥样品中重金属含量的检测,计算地积累指数(Igeo),得出友谊河底泥中的Hg、Zn和Cu分别为严重污染,重污染和偏重污染水平,这可能是影响藻类光合作用的主要污染物.本研究为进行底泥毒性评估提供了新的原位分析方法。New modulated chlorophyll fluorescence imaging system Imaging-PAM has been applied as an effective detection in short-term bioassays with Scenedesmus obliquus.Maximum quantum yield of chlorophyll fluorescence（Fv/Fm） and the actual fluorescence quantum yield（YⅡ） were used to assess the toxicity of the sediment from Youyi River.Data showed that in 12h exposure there was a rapid increase in Fv/Fm and YⅡof Scenedesmus obliquus in the sediment suspension of Youyi River,whereas after 12h a time-response curve appeared.In 72h the Fv/Fm and YⅡdropped to 44% and 35% of that of the control respectively,the halfinhibition time of Fv/Fm and YⅡwere 57h and 45h.At the same time by detecting the concentration of heavy metals in the sediment of Youyi River,the index of geoaccumulation（Igeo） was accounted as follows：Hg,Zn,and Cu reached serious pollution,heavy pollution and partial heavy pollution levels respectively.They are the probobly the primary contaminants affecting the photosynthesis of algae.This study may provide a new method to assess the toxicity of sediment in situ.

关 键 词：斜生栅藻 底泥毒性 叶绿素荧光量子产量 Imaging-PAM 

分 类 号：X830[环境科学与工程—环境工程]