硫酸奈替米星脂质体的制备及性质研究  

Preparation and characterization of netilmicin sulfate liposomes

作　　者：汤玥[1] 李谦[1] 谢开智[2] 洪启梅[1] 赵铮[1] 班莹[1] 何怡[1] 

机构地区：[1]中国药科大学,南京210009 [2]福建省莆田学院附属医院,莆田351100

出　　处：《中国新药杂志》2010年第21期1939-1943,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09310-004);中央高校基本科研业务费专项资金资助(JKP2009009)

摘　　要：目的:制备硫酸奈替米星脂质体并对其体内外性质进行研究。方法:采用复乳-超声法制备硫酸奈替米脂质体,采用透析法测定其包封率,并研究其渗漏率和肾组织分布。结果:制得的脂质体粒径大小均匀,包封率最高可达82.7%,最低可达53%;脂质体在4℃储存90 d渗漏率小于6%;制备成脂质体剂型可降低肾组织分布。结论:复乳-超声法可用于制备硫酸奈替米星脂质体;透析法可用于其包封率的测定;所制备的脂质体较稳定,可降低肾毒性。Objective： To prepare netilmicin sulfate liposomes and characterize the liposomes.Methods：Netilmicin sulfate was encapsulated into the liposomes using ultrasound-assisted multiple emulsification technique.Dialysis was used to determine the entrapment efficiency.Release percentage and distribution in kidney was studied.Results：The sizes of netilmicin sulfate liposomes were well-proportioned.The entrapment efficiency of netilmicin sulfate liposomes was in the range of 53% to 82.7%.The release percentage of the liposomes was lower than 6% when storage under 4 ℃ after 3 months.The liposomes had lower targeting efficiency in mouse kidney after iv administration as compared with netilmicin sulfate solution.Conclusion：Ultrasound-assisted multiple emulsification technique is feasible to prepare netilmicin sulfate liposomes,and dialysis is fit for determining the entrapment efficiency.Netilmicin sulfate liposomes are stable and may have the potential to reduce adverse reactions.

关 键 词：硫酸奈替米星脂质体 高效液相色谱法 制备 性质 

分 类 号：R943.4[医药卫生—药剂学] R978.12[医药卫生—药学]