养阴清热方对615小鼠HCa-F肝癌端粒酶活性的影响  

The Effect of Telomerase Activity of Chinese Medicine ATU-Ⅰ on 615 Mice with HCa-F Liver Carcinoma

作　　者：谢长生[1] 高文仓[2] 吴良村[1] 

机构地区：[1]浙江省中医院肿瘤科,浙江杭州310006 [2]大连大学医学院附属医院肿瘤科,辽宁大连116021

出　　处：《中华中医药学刊》2010年第11期2324-2326,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药管理局基金资助项目(2006C029)

摘　　要：目的:探讨复方中药抗肿瘤的机制及作用靶点。方法:造模后,荷瘤鼠随机分成6组,分别给生理盐水(空白组)、养阴清热方——安体优Ⅰ号低(ATU-L)、中(ATU-M)、高(ATU-H)3个剂量组灌胃、及阳性对照α-IFN和紫杉醇(PTX)组治疗,第14、21天处死动物,制备相应标本,以聚丙烯酰胺凝胶电泳—银染法及TRAP-PCR-ELISA法,定性、定量测定端粒酶活性。结果ATU-H组及ATU-M组对荷瘤小鼠的端粒酶活性有一定抑制作用,在治疗第14天及第21天,ATU-H组端粒酶活性均为阴性,ATU-L组均为阳性,而ATU-M组在第14天为阳性,在第21天为阴性,电泳银染法也显示了相近结果。结论:安体优Ⅰ号对615小鼠HCa-F肝癌的端粒酶活性具有一定的抑制作用。Objective：To discover the anti-tumor mechanism and action target of Traditional Chinese Medicine ATU-Ⅰ on the basis of observing its inhibition of subcutaneous transplanting liver carcinoma（HCa-F） of recombinant in-bred strain 615 mice.Methods：All the tested carcinoma model mice are grouped into 6 groups which were administered with N.S.（model group/negative control）,AUT-I low dose（ATU-L）,AUT-I medium dose（ATU-M）,AUT-I high dose（ATU-H）,（-IFN（positive control 1）,and Pacilitaxel（positive control 2） respectively.Keep observing the change of tumor volume and survival time until the 14thand 21st day when some of the tested mice were killed,tumors were weighed,and the relative sample were made respectively.The activity of telomerase was measured qualitatively and quantitatively through PAGE-silver-staining assay and TRAP-PCR-ELISA assay.Results ：The activity of telomerase was inhibited in ATU-H group and ATU-M group.On day 14 and day 21 of administration,research has showed that the telomerase activity was negative in ATU-H group and positive in ATU-L group.Whereas ATU-M group,it showed positive on day 14 and negative on day 21.Similar result was shown with PAGE-silver-staining assay.Conclusions： ATU-I can partly inhibit the activity of telomerase of 615 mice with HCa-F liver carcinoma.

关 键 词：中医药疗法/养阴清热方 端粒酶/端粒酶抑制 

分 类 号：R285.5[医药卫生—中药学]