骨髓间充质干细胞联合甲基强的松龙防治百草枯中毒急性肺损伤的实验研究  

Study of bone marrow mesenchymal stem cells and methylprednisolone on paraquat-induced acute lung injury in rats

作　　者：黄杨[1] 贾斌[2] 尹文[1] 余厚友[1] 王玉同[1] 郝璐[1] 刘健[1] 

机构地区：[1]第四军医大学西京医院急诊科,陕西710033 [2]西北工业大学生命科学院实验中心

出　　处：《中国急救复苏与灾害医学杂志》2010年第11期1018-1020,1029,共4页China Journal of Emergency Resuscitation and Disaster Medicine

基　　金：基金项目：陕西省自然科学基金资助项目（2010JM4051）

摘　　要：目的通过移植骨髓间充质于细胞（BMSCs）联合甲基强的松龙（MP），观察其对百草枯（PQ）中毒急性肺损伤的防治效果。方法54只雌性受体大鼠随机分为5组：PQ组、BMSCs组、MP组、BMSCs＋MP组和正常对照组，观察7d内各组大鼠的肺湿干比、血浆中IL-18、TNF-α、MDA、SOD的水平变化。结果与PQ组相比，BMSCs组和MP组能明显降低肺湿干比（P≤0．01）和炎症介质IL-1β、TNF—α的含量（P≤0．01），降低血浆中MDA水平（P≤0．01）和升高SOD水平（P≤0．01）。但BMSCs＋MP组的上述指标均显著优于BMSCs组和MP组（P≤0．01）。结论BMSCs与MP的联合应用，既起到抑制炎症反应、减少脂质过氧化产物的协同作用，又促进移植BMSCs的体内存活，增强了移植效果。Objective To investigate the protective effects of bone marrow mesenchymal stem cells （BMSCs） and methylprednisolone （MP） on Paraquat（PQ）-induced acute lung injury in rats. Methods 54 female recipient SD rats were divided randomly into five groups- PQ group, BMSCs group, MP group, BMSCs＋MP group and normal control group. The lung wet/dry weight ratios were calculated during 7 days after injections in each grouP. At the same time, the levels of Interleukin-1 β （IL-1β）, tumor necrosis faetor-α （TNF-α）, malondialdehyde ＇（MDA）, superoxide dismutase （SOD） in plasma were detected. Results Compared with PQ group, the lung wet/dry weight ratios were down obviously in BMSCs group and MP group, at the same time, the levels of IL-1β, TNF-α, and MDA in plasma were decreased markedly, and the activities of SOD in plasma were increased significantly in the both groups. However, the detective results in BMSCs ＋ MP group were significantly better than those in BMSCs group and MP group. Conclusion BMSCs and MP applications can not only restrain inflammatory reaction and reduce lipid peroxide, but also enhance the effect of BMSCs transplantation.

关 键 词：百草枯 骨髓间充质干细胞 甲基强的松龙 急性肺损伤 

分 类 号：R595.4[医药卫生—内科学]