地锦草等中药提取物对大肠杆菌耐药消除作用的初步研究  

Primary Study on Extractions from Herba euphorbiae humifusae to Eliminate Resistance of Escherichia coli

作　　者：高迎春 魏秀丽 张传津 苏梅 杨修镇 肖永霞[2] 都业良[2] 

机构地区：[1]山东省兽药质量检验所,济南250022 [2]青岛农业大学,山东青岛266109

出　　处：《中国兽药杂志》2010年第11期12-16,共5页Chinese Journal of Veterinary Drug

基　　金：十一五国家科技支撑计划重大项目"抗耐药菌中兽药的研制与开发"(2008BADB4B04)的子项目"中药耐药抑制剂地锦草提取物及其制剂的研制与开发"(2008BADB4B04-5)

摘　　要：对动物源大肠杆菌采用二倍稀释法对氨基糖苷类药物及其他药物进行了MIC的测定,结果显示对十种西药全部耐药。应用1/2MIC的中药提取物对耐药菌株进行了耐药性消除的初步探索研究,结果表明临床多重耐药大肠杆菌用中药提取物作用24代以后,地锦草提取物耐药消除最强,三黄汤提取物次之,对某些氨基糖苷类药物的MIC明显下降。另外,对消除前后的细菌提取质粒,扩增氨基糖苷类的耐药基因,发现随着MIC的降低,耐药基因的数量也不同程度地减少和消失。由此可见地锦草等中药提取物对可移动的耐药基因元件有一定的消除作用,对耐药基因的扩散有抑制作用。Drug sensitivity tests with double dilution method for E.coli strains that isolated from animals showed they had obvious resistance to 10 of 10 kinds of antibiotics tested.With extractions from Herba euphorbiae humifusae and other Herba acting on resistant E.coli to eliminate drug resistance,result showed that extractions from Herba euphorbiae humifusae have best elimination,the resistance to aminoglycoside antibiotics decline;extracting plasmid of E.coli,amplification aminoglycoside resistant gene,founding as the decline MIC,aminoglycoside resistant gene also decline to a certian extent.As a result extractions from Herba euphorbiae humifusae could eliminate removable drug resistance gene and control resistance prevalence.

关 键 词：耐药性 地锦草提取物 消除作用 大肠杆菌 

分 类 号：S853.72[农业科学—临床兽医学]