HPLC测定九味羌活丸中黄芩苷、甘草酸和异甘草素含量  

Determination of baicalin, glycyrrhizic acid and isoliquiritigenin in Jiuwei Qianghuo Pill by HPLC

作　　者：宋九华[1] 王刚[1] 刘素君[1] 

机构地区：[1]乐山师范学院化学与生命科学学院天然产物研究所,四川乐山614004

出　　处：《中成药》2010年第11期1914-1916,共3页Chinese Traditional Patent Medicine

基　　金：国家十一五科技支撑计划项目(2007BAC18B04)

摘　　要：目的:建立九味羌活丸(羌活、防风、苍术、细辛、川芎、白芷等)中黄芩苷、甘草酸和异甘草素的含量测定方法。方法:色谱柱:Kromasil ODS1(200 mm×4.6 mm,5.0μm)。流动相:甲醇-0.5%冰醋酸梯度洗脱;流速:1 mL/min;检测波长:0～8 min为276 nm,8～15 min为370 nm,15 min以后为250 nm。结果:黄芩苷在0.155 0～3.100 0μg范围内与峰面积呈良好的线性关系,r=1.000 0,平均回收率99.23%,RSD为0.52%。甘草酸在0.195 9～3.918 0μg范围内与峰面积呈良好的线性关系,r=1.000 0,平均回收率98.81%,RSD为0.53%。异甘草素在0.012 4～0.248 0μg范围内与峰面积呈良好的线性关系,r=1.000 0,平均回收率99.11%,RSD为0.72%。结论:所建立的方法简便可行、重现性好、可作为九味羌活丸的质量控制。AIM：To develop an HPLC method for determining baicalin,glycyrrhizic acid and isoliquiritigenin in Jiuwei Qianghuo Pill（Rhizoma et Radix Notopterygii,Radix Saposhnikoviae,Rhizoma Atractylodis,Radix et Rhi-zoma Asari,Rhizoma Chuanxiong,Radix Angelicae dahuricae,etc.）.METHODS：The column Kromasil ODS1（200 mm ×4.6 mm,5.0 μm）was used.The mobile phase consisted of methanol-0.5% acetic acid with a gradient elution,the flow rate was 1.0 mL/min,and the detection wavelength respectively was set at 276 nm in the range of 0-8 min,370 nm in the range of 8-15 min,250 nm in the range of 15-40 min.RESULTS：The linear range of ba-icalin was 0.155 0-3.100 0 μg,r = 1.000 0.The average recovery was 99.23%（RSD = 0.52%）.The linear range of glycyrrhizic acid was 0.195 9-3.918 0 μg,r = 1.000 0.The average recovery was 98.81%（RSD = 0.53%）.The linear range of isoliquiritigenin was 0.012 4-0.248 0 μg,r = 1.000 0.The average recovery was 99.11%（RSD =0.72%）.CONCLUSION： This method is convenient and reproducible.It can be used for the quality control in production of Jiuwei Qianghuo Pill.

关 键 词：九味羌活丸 黄芩苷 甘草酸 异甘草素 HPLC 

分 类 号：R927.2[医药卫生—药学]