高氧暴露对早产儿血单个核细胞血红素加氧酶-1表达的影响  

The effect of hyperoxia-exposure on the expression of heme oxygenase-1 in the PBMC of premature infants

作　　者：全裕凤[1] 郑明慈[1] 张华[1] 欧维林[1] 张培林[1] 

机构地区：[1]桂林医学院附属医院新生儿科,广西桂林541001

出　　处：《华夏医学》2010年第5期466-469,共4页Acta Medicinae Sinica

基　　金：广西科技厅科技攻关基金资助项目(0472002-35);广西卫生厅医药卫生基金资助项目(Z2001061)

摘　　要：目的:观察血红素加氧酶-1(heme oxygenase-1,HO-1)在高氧暴露早产儿血单个核细胞HO-1蛋白及mRNA的表达水平。方法:分别用反转录聚合酶链反应技术、Western Blot对40例吸入高氧的早产儿(高氧组)血中单个核细胞HO-1 mRNA表达水平及蛋白表达水平进行检测,并与常压空气的早产儿40例对照(对照组)。结果:与对照组相比,高氧组HO-1 mRNA、HO-1蛋白表达明显增加(P<0.001,P<0.01)。结论:高氧暴露可上调早产儿血单个核细胞血红素加氧酶-1的表达,HO-1可能参与了早产儿高氧肺损伤。Objective： To observe the protein and mRNA expression of HO-1 in the peripheral blood mononuclear cell（PBMC） of the hyperoxia-exposed premature infants.Methods： The expressions of HO-1 mRNA and protein in the PBMC of 40 threeday-old hyperoxia-exposed preterm infants（hyperoxia group） and 40 airexposed preterm infants（control group） were measured by reverse transcription polymerase chain reaction（RT-PCR） and Western blot respectivesly.Results： Comparing with the aasay results of the control group,the protein expression of HO-1,mRNA,HO-1 in the hyperoxia group was significantly increased（P〈0.001 and 0.01 respectivesly）.Conclusion： Hyperoxia may upregulate the expression of HO-1 in PBMC of hyperoxia-exposed premature infants,and HO-1 may be associated with the hyperoxia-induced lung injury of premature infants.

关 键 词：血红素加氧酶-1 高体积分数氧 婴儿 早产儿 

分 类 号：R722.6[医药卫生—儿科]