HPLC-PDA法检测西红花和红花中十一种非法添加色素  

Determination of eleven colouring agent in Saffron and Safflower with HPLC-PDA

作　　者：邹耀华[1] 殷红妹 郭怡飚 宋旭峰[1] 

机构地区：[1]浙江省杭州市药品检验所,杭州310017

出　　处：《中国卫生检验杂志》2010年第11期2724-2725,2728,共3页Chinese Journal of Health Laboratory Technology

基　　金：杭州市科技发展计划项目(20090833B31)

摘　　要：目的:建立HPLC-PDA法测定西红花和红花中非法添加色素苋菜红、胭脂红、金橙G、日落黄、诱惑红、丽春红2R、酸性红73、金橙Ⅱ、赤藓红、新红、808猩红的方法。方法:样品用70%乙醇提取,以甲醇-0.05 mol/L醋酸铵溶液梯度洗脱,二极管阵列检测器检测。结果:该方法检出限为0.90 mg/kg～1.50 mg/kg,2档添加回收率为94.6%～102.5%;市售西红花、红花中多批次检出金橙Ⅱ、胭脂红、日落黄及诱惑红。结论:本实验采用HPLC-PDA法同时检测西红花和红花中十一种常用的非法添加色素,方法简单、准确、快速,可以为制药企业或药品监督管理部门控制西红花和红花的质量提供一定的参考。Objective：A method for rapid determination of eleven colouring agent in Saffron and Safflower（included Amaranth,Ponceau 4R,Orange G,Sunset Yellow,Allura red,Ponceau 2R,Acid Red 73,Orange II,Pyrosine,New red,808 Scarlet） was developed.Methods： The sample was extracted by 70% ethanol,gradient elution with methanol and 0.05 mol/L ammonium acetate and then detected by Photo-Diode Array Detector.Results： The limits of quantitation（LOQs） of the method were 0.90 mg/kg～1.50 mg/kg,and fortified recovery of reference substance solutions at two levels were 94.6%～102.5%,the RSDs were below 2.2%（n=3）.Eleven reference substance solutions were separated well in forty five minutes.Many colouring agent were detected in Saffron and Safflower as Ponceau 4R,Sunset Yellow,Allura red and Orange II.Conclusion： This method is simple,rapid,and reproducible,and it can be used as a low-cost, convenient method for measuring colouring agent in Saffron and Safflower,and it can provide reference or help for relative company and State food and drug administration.

关 键 词：高效液相色谱 二极管阵列检测器 西红花 红花 色素 

分 类 号：O657.72[理学—分析化学]