监测乙肝病毒表面抗原大蛋白水平评价乙肝诊疗状态  

Monitoring levels of HBV-LP for the assessment of diagnosis and treatment in the patients with Hepatitis B

作　　者：单平囡[1,2] 卢志勇[1,2] 宣国红[1,2] 茹金城[1,2] 许德顺[1,2] 

机构地区：[1]绍兴县中心医院 [2]中国医科大学绍兴医院检验科,浙江绍兴312030

出　　处：《中国卫生检验杂志》2010年第11期2942-2944,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:检测乙肝病毒表面抗原大蛋白水平的变化,探讨其对乙肝病人诊疗状态的评价价值。方法:1042例乙肝病人以ELISA法进行BHM分组,分别对"大三阳"组、"小三阳"组、HBeAg阴性组、HBV DNA检测阴性组和HBV基因变异组进行HBV-LP测定(ELISA法)及HBV DNA检测(FQ-PCR法)。结果:1042例乙肝病人检测结果显示:L、S、EN及DN组HBV-LP与HBV DNA阳性率分别为73.13%、67.96%,72.73%、42.32%和60.40%、39.88%,除L组(P>0.05)外,其余各组相比差异显著有统计学意义(P<0.01,P<0.05);两种检测方法之间有很好的正相关性(r=0.8420);对HBV DNA检测阴性的196例标本HBV-LP检测的阳性率为68.88%。结论:HBV-LP检测可作为从蛋白水平反映HBV病人体内病毒复制程度的血清学指标,对于HBeAg阴性的乙肝病人HBV-LP检测反映病毒复制优于HBV DNA检测,并与之具有很好互补作用。Objective：To determinate hepatitis B large surface protein（HBV-LP）for investigating the evaluation value of HBV-LP on the patients with hepatitis B.Methods： Among the groups of L,S,EN and DN HBV-LP（by ELISA） and HBV DNA（by FQ-PCR） were measured respectively in 1042 patients with hepatitis B.Results： There were significant difference between the positive expression rates of HBV-LP and HBV DNA in the groups of S（72.73%,42.32%,P0.01）,EN（60.40%,39.88%,P0.005） and DN（68.88%） and showed a higher positive correlation（r=0.8420） except comparing between the L group（73.13%,67.96%,P0.05）.Conclusion： The HBV-LP detecting would be a serological index that reflect probably the replication of HBV at the protein level,which had an advantage and complementary action between HBV DNA detecting for HBeAg negative patients.

关 键 词：乙肝病毒表面抗原大蛋白 病毒复制 血清学指标 HBEAG阴性 

分 类 号：R373.2[医药卫生—病原生物学]