舒马曲坦对偏头痛模型大鼠中脑导水管周围灰质区核转录因子-κB表达的影响  

The influence of NF-κB protein expression in the periaqueductal gray matter induced by sumatriptan in migraine animal model

作　　者：王蓉飞[1] 于生元[1] 

机构地区：[1]解放军总医院神经内科,北京100853

出　　处：《中华神经科杂志》2010年第10期725-728,共4页Chinese Journal of Neurology

基　　金：国家自然科学基金资助项目（30570264）

摘　　要：目的 探讨舒马曲坦治疗偏头痛的作用机制.方法 应用电刺激大鼠上矢状窦区硬脑膜制作偏头痛动物模型,观察给予舒马曲坦后中脑导水管周围灰质核转录因子-κB（NF-κB）蛋白表达的变化.结果 空白组、假手术组、电刺激组、舒马曲坦组、生理盐水对照组每张切片的NF-κB阳性神经元计数分别为111.7±15.7、112.9±10.7、508.7±30.8、179.5±14.9、497.8±20.6.刺激组与空白组、假手术组及舒马曲坦组比较,差异有统计学意义（F=944.78,P〈0.05）.结论 电刺激大鼠上矢状窦区硬脑膜后,在中脑导水管周围灰质区出现了NF-κB细胞的激活,舒马曲坦可使其表达降低.Objectiye To investigate the possible mechanism of sumatriptan in treating migraine.Methods Migraine model rat was electrically stimulated at cerebral dura mater in superior sagittal sinus （SSS） to induce migraine.Each of 10 male SD rats were randomly selected into groups of normal control （no intervention）, pseudo-stimulation group （ no electrical stimulation）, stimulation group （ no treatment after stimulation）, saline group （treated with saline after migraine stimulation ） and sumatriptan group （ treated with sumatriptan after migraine stimulation）.The neurons expressing NF-κB in periaqueductal gray matter （PAG） were evaluated in each group by immnunostaining.Results The numbers of NF-κB immunoreactive positive neurons were 179.5 ± 14.9 in sumatriptan group, 497.8 ± 20.6 in saline group,508.7 ± 30.8 in stimulation group, 112.9 ± 10.7 in pseudo-stimulation group and 111.7 ± 15.7 in normal group.The stimulation group was higher than normal group, pseudo-stimulation group and sumatriptan group （F=944.78, P 〈0.05）.Conclusions The NF-κB positive neurons were activated in the PAG by electric stimulation of the dura mater near the SSS.Sumatriptan can reduce the expression of NF-κB protein.

关 键 词：偏头痛 水管周灰质 NF—κB 舒马普坦 

分 类 号：R686[医药卫生—骨科学]