引起乌鲁木齐成人麻疹暴发的麻疹病毒基因特征分析  

Genetic characterization of measles virus caused the measles outbreak in Xinjiang

作　　者：米吉提·买买提[1] 王慧玲[2,3] 许松涛[2] 毛乃颖[2] 许文波[2] 张燕[2] 

机构地区：[1]新疆维吾尔自治区人民医院,乌鲁木齐830001 [2]中国疾病预防控制中心病毒病预防控制所麻疹室 [3]北京儿童医院

出　　处：《中华实验和临床病毒学杂志》2010年第5期337-339,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：新疆维吾尔自治区乌鲁木齐市科学技术计划项目（Y08231011）

摘　　要：目的 分析引起新疆乌鲁木齐市2008年成人麻疹暴发流行的麻疹野病毒的基因特征.方法 用Vero/Slam细胞从成人麻疹暴发患者的标本中分离出麻疹野病毒,应用逆转录-聚合酶链反应（RT-PCR）对麻疹野病毒分离株扩增核蛋白N基因羧基末端676个核苷酸片段,再对扩增产物进行核苷酸序列测定和分析.以N基因羧基末端450个核苷酸片段构建基因亲缘性关系树,进行核苷酸变异分析.结果 共分离到11株麻疹野病毒,这些病毒N基因450个核苷酸之间的差异为0～0.2%,均为H1基因型中的H1a基因亚型.11株麻疹野病毒与H1基因型代表株（Chin9372）的核苷酸和氨基酸差异分别为：2.2%～2.4%之间和4.0%～4.6%之间.结论 引起新疆乌鲁木齐市2008年成人麻疹暴发流行的麻疹野病毒为H1a基因亚型.Objective To analyze the genetic characterizations of wild type measles viruses caused the measles outbreak in Xinjiang. Methods Vero/Slam cell were used for measles viruses isolation from the specimens collected from measles outbreaks patients. Fragment of 676 nucleotide acids of the carboxylend of nucleoprotein gene were amplified using reverse transcription-polymerase chain reation （RT-PCR） method and then the PCR products were directly sequenced and analyzed. Phylogenetic tree was constructed based on 450 nucleotide acids of the N-terminus of nucleoprotein gene, and homological analysis was performed at nucleotide acid level. Results 11 measles viruses were sequenced and all belonged to H1a subgenotype.The nucleotide difference was 0-0. 2% between 11 Xinjiang isolates. And the nucleotide difference was 2.2%-2.4% between Xinjiang isolates and H1 genotype reference strain. Conclusion The Measles viruses causing the measles outbreak in Xinjiang were H1a subgenotype.

关 键 词：麻疹病毒 序列分析 基因型 

分 类 号：R511.1[医药卫生—内科学]