乙型脑炎野毒株和弱毒株（SA14-14-2）在豚鼠体内的病毒血症比较试验  

Comparision of viremia formation between guinea-pigs infected with wild and attenuated （ SA14-14-2） Japanese encephalitis viruses

作　　者：刘欣玉[1] 俞永新[1] 徐宏山[1] 梁国栋[2] 王环宇[2] 贾丽丽[1] 董关木[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]中国疾病预防控制中心病毒病预防控制所病毒性脑炎室

出　　处：《中华实验和临床病毒学杂志》2010年第5期343-345,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 利用豚鼠病毒血症的产生强度对乙型脑炎野毒株和弱毒株病毒毒力进行评价.方法 以不同乙脑野毒株和减毒株及其母株病毒分别接种豚鼠,观察不同时间产生病毒血症的水平.结果 接种后1 d和3 d,不同乙脑野毒株均可检测到不同水平的病毒血症（1.00～3.40 lg pfu）,以相同滴度（104pfu）的病毒接种豚鼠时,疫苗株母株SA14产生较高水平的毒血症（2.41～3.40 lg pfu）并至少持续3 d,而SA14-14-2疫苗株未产生毒血症.结论 豚鼠病毒血症的产生强度可以作为一种新的方法对乙型脑炎病毒的减毒和疫苗株的毒力进行评价.Objective To study the viremia formation in guinea-pigs infected with wild type and attenuated Japanese encephalitis virus （JEV）. Methods Guniea pigs were inoculated intraperitoneally with different wild JEV strains and the attenuated vaccine strain and its parent virulent strain. Viremia was detected on different days following virus inoculation. Results All the guinea-pigs inoculated with the wild JEV strains induced different levels of viremia （ 1.00-3.40 lg pfu） on the 1 st and 3rd day post inoculation.Using a virus titer of 104 pfu for inoculation, the animals inoculated with the SA14 parent strain induced relatively high viremia （1024-10 3.4 pfu）, however no viremia coulds be detected on any tested days.Conclusion The degree of viremia in guinea pigs can be used as a new method to evaluate the attenuation of JEV.

关 键 词：脑炎 日本 毒力 模型 动物 毒血症 

分 类 号：R373[医药卫生—病原生物学]