血清乙型肝炎病毒大蛋白检测及其临床意义  

Clinical significance of measuring hepatitis B virus large surface protein in serum

作　　者：王念跃[1] 张岱[2] 葛彦文[3] 赵伟[1] 

机构地区：[1]东南大学附属第二医院,南京210003 [2]河南中医药大学第一附属医院 [3]江苏大学医学技术学院

出　　处：《中华实验和临床病毒学杂志》2010年第5期349-351,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 通过检测患者血清乙肝病毒外膜大蛋白（Hepatitis B virus large protein,HBVLP）、HBV-DNA以及乙肝病毒标志物（HBV-M）,探讨HBV-LP对于反映体内乙肝病毒复制的意义.方法 对1886份血清标本采用酶联免疫吸附试验检测HBV-LP;化学发光免疫法测定HBV-M含量,PCR实时荧光定量检测HBV-DNA.结果 乙肝患者大蛋白检测结果与HBV-DNA检测结果差异无统计学意义（x2=1.142,P＞0.05）.HBV-DNA拷贝数的对数值与HBV-LP吸光度呈正相关（r=0.487,P＜0.01）,在不同HBV-DNA拷贝数组别间,HBV-LP A值差异有统计学意义（F=7.772,P＜0.01）.在不同HBV-M模式中,HBV-DNA与HBV-LP的检测阳性率差异均无统计学意义.36例健康对照者,其HBV-DNA及HBV-LP测定结果均为阴性.结论 血清中HBV-LP的吸光密度值与HBV-DNA的拷贝数的对数有较好的相关性,HBV-LP能够反应HBV的复制情况.Objective To explore the significance of serum hepatitis B virus large protein（ HBVLP） , HBV-DNA and markers of hepatitis B virus （HBV-M）in the diagnosis of viral replication. Methods Serum HBV-DNA level was quantitantively detected using PCR Real-time polymerase chain reaction, HBVLP was detected by enzyme-linked immunosorbent assay （ELISA） and HBV markers expression were measured by chemiluminescence immunoassay method in 1886 cases of seurm. Results The results of hepatitis virus large protein （HBV-LP） detection and the detection results of HBV-DNA was no significant difference （x2 = 1. 142,P ＞0. 05）. HBV-DNA logarithm of copies and A vaule of HBV-LP was a positive correlation （r = 0. 487, P ＜ 0.01 ）. HBV-DNA copies of different groups was significantly different from HBV-LPA values （ F = 7. 772, P ＜ 0. 01 ）. The results of HBV-LP and HBV-DNA detected in different patterns of HBV-M were not significantly different. In 36 healthy people,the detecting results of HBV-DNA and HBV-LP are negetive. Conclusion There is a good correlation between the copies of HBV-DNA and the levels of HBV-LP. HBV-LP expression can reflect the replication of HBV.

关 键 词：肝炎 乙型 病毒包膜蛋白质类 DNA 生物学标记 

分 类 号：R512.62[医药卫生—内科学]