感染EB病毒的传染性单核细胞增多症患儿外周血白细胞CCR3和CCR5的表达  

The expression of periphery blood leucocyte CCR3 and CCR5 in the children with Epstein-Barr virus associated infectious mononucleosis

作　　者：齐铁雄[1] 高国花[2] 刘世华[1] 

机构地区：[1]航空工业中心医院儿科,北京100012 [2]华北石油管理局总医院儿科

出　　处：《中华实验和临床病毒学杂志》2010年第5期355-357,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 探讨感染EB病毒的传染性单核细胞增多症（IM）患儿CCR3和CCR5的表达,以期了解Th1/Th2细胞的分化情况.方法 观察患儿外周血异型淋巴细胞比例,测定患儿的嗜异凝集抗体,用双抗体夹心酶联免疫吸附法（ELISA）测定患儿抗EBV-CA-IgM、抗EBV-CA-IgG及抗EBV-NA-IgG,筛选出符合诊断标准的IM患儿.并用流式细胞检测仪检测淋巴细胞中CCR3和CCR5的表达.结果 IM感染组异型淋巴细胞比例高于对照组（P＜0.05）.IM感染组CCR3＋细胞率高于对照组（P＜0.05）,CCR5＋细胞率低于对照组（P＜0.05）.CCR3与发热持续时间、异型淋巴细胞百分数呈正相关（P＜0.05）.CCR5与发热持续时间呈负相关（P＜0.05）.结论 IM患儿CCR3＋表达增高,CCR5＋表达降低,存在以Th2细胞优势分化状态为特征的T辅助细胞分化失衡.CCR3和CCR5可以作为判断IM病情轻重的重要参考指标.Objective To explore the expression of periphery blood leucocyte CCR3 and CCR5 and to comprehend T helper cell in the Children with Epstein-Barr virus associated infectious mononucleosis.Methods We defined the children according to the diagnosis criterion through Paul-Bunnell test inspecting the children＇s periphery blood unusual lymphocyte and detecting their anti-EBV-CA-IgM, anti-EBV-CA-IgG and anti-EBV-NA-IgG by ELISA and counted the ratio of CCR3 ＋ and CCR5 ＋ cells in lymphocytes with flow cytometry. Results The ratio of unusual lymphocyte in IM was higher than that of the healthy control group （P ＜ O. 05）. The ratio of CCR3 ＋ cells in IM group was higher than that of the healthy control group （P ＜ 0.05）. The ratio of CCR5 ＋ cells in IM group was significantly lower than that of the healthy control group. CCR3 ＋ had direct interrelation with fever continued time and the ratio of unusual lymphocyte. There was a negative interrelation between CCR5 and fever continued time （ P ＜ 0. 05 ）. Conclusions Children infectious of IM expressed higher level of CCR3 ＋ and lower level of CCR5 ＋ and there was a tendency of Th2 polarization with over production of T helper cell divide imbalance. CCR3 ＋ and CCR5 ＋ may be important targets to judge the degree of seriousness of IM.

关 键 词：疱疹病毒4型 人 传染性单核细胞增多症 受体 CCR3 受体 CCR5 T淋巴细胞亚群 

分 类 号：R725.1[医药卫生—儿科]