鲤胰岛素样生长因子结合蛋白-2和-3基因启动子克隆与序列分析  

Cloning and analysis of insulin-like growth factor binding protein-2 and -3 promoters in common carp(Cyprinus carpio)

作　　者：陈文波[1,2] 李文笙[1] 林浩然[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室,水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室,广东广州510275 [2]河南理工大学资源环境学院,河南焦作454000

出　　处：《水产学报》2010年第10期1469-1477,共9页Journal of Fisheries of China

基　　金：国家“九七三”重点基础研究计划(2004CB117402,2010CB126302);国家自然科学基金项目(30671626);广东省科技计划项目(2007B030300001)

摘　　要：胰岛素样生长因子(insulin-like growth factors,IGFs)系统对鱼类的生长和繁殖有着重要的作用。利用PCR方法克隆获得了鲤胰岛素样生长因子结合蛋白(insulin-like growth factor binding protein,IGFBP)-2和-3基因的上游启动子部分序列,长度分别是1142bp和1067bp。Igfbp-2启动子区域没有TATA框和CAAT框。同时,Igfbp-3中只含有TATA框,但没有CAAT框。研究结果表明,二者启动子不具备典型的启动子特征,同时,在二者启动子上还发现了cAMP应答元件和肝细胞核因子结合位点,以及Pit-1、Oct-1、RXR和GR等结合位点。研究认为,鲤Igfbp-2和-3基因的表达受到潜在的多因子调控。Insulin-like growth factors（IGFs）system,including IGFs and IGF-binding proteins,plays a central role in growth and reproduction of fish and higher vertebrates.In serum and extracellular fluids,most circulating IGFs are bound to specific high affinity IGF-binding proteins（IGFBPs）that can protect them from degradation and modulate their actions.Common carp（Cyprinus carpio）is one of the most economically important fish in China.To improve the yield of common carp and get more economic benefits,the study of mechanism of growth and reproduction is very important.In the previous study,the cDNAs of common carp Igfbp-2 and-3 had been cloned,and the expression profiles and hormonal regulation were studied.The results showed that common carp Igfbp-2 and-3 mRNAs were expressed in many various tissues and different embryonic developmental stages,but the abundances were different significantly.To further understand the regulation mechanism of Igfbp-2 and-3,the study of their promoters is necessary.So,in this paper,the sequences of Igfbp-2 and-3 promoters were firstly cloned,and then the putative transcription binding sites were analyzed.The length of Igfbp-2 and-3 promoters we obtained was 1 142 bp and 1 067 bp,respectively.There was a consensus TATA box 22 bp 5＇ to the start site of transcription in Igfbp-3 promoter,while in the upstream there was a SP1 binding site instead of CAAT box.Neither TATA box nor CAAT box was found in the promoter of Igfbp-2,only a SP1 binding site and rich G/C motif in this region.These results showed that the promoters of common carp Igfbp-2 and-3 did not contain the characteristics of classic promoter.Furthermore,some other transcription binding sites were observed in the two promoters,such as CREB,HNF,Pit-1,Oct-1,RXR,GR,ER,PR,SRY and so on,while the STAT（signal transducer and activator of transcription）was only found in Igfbp-3.These results suggested that there were basic and hormone-inducible expression patterns of Igfbp-2 and-3 genes.However,the regulation mechanisms were n

关 键 词：鲤 胰岛素样生长因子结合蛋白 启动子 序列分析 

分 类 号：Q785[生物学—分子生物学] S917[农业科学—水产科学]