第四代凡纳滨对虾抗WSSV选育家系的抗病及免疫特性研究  

Studies on WSSV-resistant and immune characteristics of the 4^(th) generation selective breeding families for resistance to the white spot syndrome virus(WSSV)of Litopenaeus vannamei

作　　者：黄永春[1,2] 艾华水[1] 殷志新[1] 黄仙德[1] 李色东 翁少萍[1] 何建国[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]集美大学水产学院,福建厦门361021 [3]广东湛江恒兴集团有限公司,广东广州524033

出　　处：《水产学报》2010年第10期1549-1558,共10页Journal of Fisheries of China

基　　金：凡纳滨对虾抗白斑综合征病毒品系选育--"南方海水养殖种子工程规模化应用技术(Ⅰ)项目(2002AA603031)

摘　　要：35个第四代凡纳滨对虾抗WSSV选育家系和未选育对虾按每克体重注射103拷贝WSSV病毒量,根据选育家系的抗病性能分3个类群:高抗性类群,成活率达24.45%±6.56%;中抗性类群,成活率为10.70%±1.41%;敏感类群,成活率为2.72%±2.76%,各类群间差异显著(P<0.01)。对分别代表高抗、中抗和敏感类群的12、7和3号家系以及未选育对虾按每克体重注射102、103、104和105拷贝WSSV,高抗性对虾在102、103、104及105感染水平下的存活率分别为100%、23.3%±3.5%、7.8%±1.9%和0%;中抗对虾分别为87.7%±3.9%、12.2%±1.9%、0%和0%;敏感对虾分别为54.4%±3.9%、2.2%±1.9%、0%和0%;未选育对虾分别为51.1%±5.1%、0%、0%和0%。在103拷贝组感染过程中免疫相关因子的变化表明,高抗对虾血液中血细胞数分别比中抗、敏感和未选育对虾提高20.7%(P>0.05),36.7%(P<0.05)和34.4%(P<0.05);PO活力上述三类对虾提高40.0%(P<0.05),76.3%(P<0.05)和63.4%(P<0.05);SOD活力分别比上述三类对虾提高31.1%(P>0.05),58.8%(P<0.05)和32.0%(P>0.05);POD活力分别比上述三类对虾提高29.6%(P>0.05),44.9%(P<0.05)和43.3%(P<0.05);血清蛋白含量分别比分别比上述三类对虾提高31.2%(P>0.05),38.7%(P<0.05)和39.3%(P<0.05),而敏感对虾和未选育对虾之间则无显著差异。结果表明,经四代选育后的高抗对虾免疫性能明显高于其他对虾,表现出良好的抗WSSV性能。In this paper,in comparison with their WSSV-resistant characteristics,35 selective breeding families of the 4th generation and no-selected shrimp of Litopenaeus vannamei were divided into three groups：relatively high resistant families,mid resistant families and susceptible families,with the average survival rates of 24.45%±6.56%,10.70%±1.41% and 2.72%±2.76%,respectively after 103 copies WSSV/g（body weight）infection,which were significantly different among the three groups（P0.01）.Injected WSSV of 102,103,104 and 105 copies WSSV/g,the survival rate of the relative high resistant family 12 was 100%,23.3%±3.5%,7.8%±1.9% and 0 %,respectively;and the survival rate of the mid resistant family 7 was 87.7%±3.8%,12.2%±1.9%,0% and 0%,respectively;and the survival rate of the susceptible family 4 was 54.4%±3.8%,2.2%±1.9%,0% and 0%,respectively;and the survival rate of non-selected was 51.1%±5.1%,0%,0% and 0%,respectively.In comparison with mid-resistant family 7,susceptible family 3 and non-selected shrimp,the THC in relatively high resistant family 12 increased by 20.7%（P0.05）,36.7%（P0.05）and 34.4%（P0.05）compared with that of the above-mentioned shrimps,respectively after 103 copies WSSV/g（body weight）infection;the PO activities increased by 40.0%（P0.05）,76.3%（P0.05）and 63.4%（P0.05）compared with that of the above-mentioned shrimps,respectively;the SOD activities compared with 31.1%（P0.05）,58.8%（P0.05）and 32.0%（P0.05）increased by that of the above-mentioned shrimps,respectively;the POD activities compared with 29.64%（P0.05）,44.9%（P0.05）and 43.3%（P0.05）increased by that of the above-mentioned shrimp s,respectively;the content of plasma protein compared with 31.2%（P0.05）,38.7%（P0.05）and 39.3%（P0.05）increased by that of the above-mentioned shrimps,respectively.However,there were no significant differences of the THC,PO,SOD,POD and content of hemolymph protein among mid-resistant family,susceptible family and non-selected shrimp.After 4 generati

关 键 词：凡纳滨对虾 白斑综合征病毒 人工感染 家系选育 免疫相关因子 

分 类 号：S941.41[农业科学—水产养殖]