扇贝养殖海区浮游生物携带AVNV的荧光定量分析  

Detection of acute viral necrosis virus(AVNV)in plankton using FQ-PCR during a molecular epidemiology survey

作　　者：王娜[1] 李赟[1] 任伟成[2] 蔡玉勇[1,2] 王崇明[2] 

机构地区：[1]中国海洋大学教育部海水养殖重点实验室,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《水产学报》2010年第10期1566-1571,共6页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2006AA100307);农业部公益项目"贝类养殖病害监测与防治技术研究"和现代农业产业技术体系建设专项资助(nycytx-47)

摘　　要：为了探明养殖海区浮游生物与AVNV流行传播的关系,实验自2009年5月至11月,分别从青岛沙子口和荣成桑沟湾两个扇贝养殖海区定期采取50和300cm两个水层水样,经25、3和0.22μm3个孔径的滤膜分级过滤收集3个粒径的浮游生物组分,利用荧光定量PCR技术对浮游生物携带AVNV的情况进行了定量检测。结果表明两个海区浮游生物样品均检测到AVNV,检测的AVNV数量也均在8月份达到峰值,沙子口海区每升海水中浮游生物携带4.11×106拷贝的AVNV,桑沟湾海区浮游生物携带1.49×105拷贝的AVNV。两个海区3个粒径浮游生物组分携带AVNV的数量从高到低依次为0.22～3μm组分、3～25μm组分和大于25μm组分,但两个水层(50和300cm)浮游生物携带AVNV的数量则没有显著差异。结合养殖期间扇贝摄食浮游生物的特点,研究认为,扇贝在养殖期间可能会通过滤食携带病毒的浮游饵料而感染AVNV,海区浮游生物可能是导致AVNV流行的重要传播媒介。AVNV（acute viral necrosis virus）is the pathogen leading to mass mortality of cultured Chlamys farreri,but the transmission of the virus is unknown.In order to clarify the relationship between the plankton and transmission of AVNV,and to afford proof for tracing transmission track,the sea water samples were fetched from 50 cm and 300 cm depths in the sea area for culturing Chlamys farreri at Shazikou of Qingdao and Sanggouwan of Rongcheng regularly from May to November in 2009,and the plankton was collected by 25 μm,3μm,0.22μm pore size filter,respectively.The AVNV carried by the plankton was identified by FQ-PCR.The results showed that the virus could be carried by the plankton,and the virus number in the plankton from both sea areas arrived at the peak in August.The highest number of AVNV identified for the samples from Shazikou sea area was 8.21×106 copies per litre sea water,and that was 2.98×105 copies for the samples from Sanggouwan sea area.Based on the number of AVNV carried,the plankton group with different particle sizes was 3-0.22 μm,25-3 μm and 25 μm from high to low in turn.The number of AVNV carried by the plankton from 50 cm and 300 cm depths was not different significantly.This is the first report about AVNV carried by the plankton from the sea area for cultured scallop.These results implied that the scallop could be infected by taking the plankton that carried AVNV as food,and the plankton was crucial carrier of AVNV.

关 键 词：栉孔扇贝 浮游生物 急性病毒性坏死症 荧光定量PCR 

分 类 号：S946[农业科学—水产养殖]