高密度脂蛋白对3T3-L1脂肪细胞分泌白细胞介素8的影响  

Effect of high-density lipoprotein on interleukin-8 secretion in 3T3-L1 adipocytes

作　　者：钟巧青[1] 赵水平[1] 王星[1] 于碧莲[1] 董静[1] 谢湘竹[1] 吴智鸿[1] 

机构地区：[1]中南大学湘雅二医院心内科,长沙410011

出　　处：《中华内分泌代谢杂志》2010年第10期888-890,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30470705）

摘　　要：分别用不同浓度高密度脂蛋白（HDL,0、10、50和100μg/ml）孵育3T3-L1脂肪细胞16 h,再加入100 ng/ml脂多糖共同孵育6 h.用酶联免疫吸附法（ELISA）检测各组脂肪细胞培养液中的白细胞介素8水平,半定量逆转录多聚酶链式反应（RT-PCR）测定脂肪细胞PPARγmRNA的表达.结果 显示,脂多糖刺激使脂肪细胞分泌白细胞介素8增加（P＜0.05）.不同浓度HDL干预的脂肪细胞分泌的白细胞介素8水平均低于脂多糖刺激组,并且呈剂量依赖性.不同浓度HDL干预的脂肪细胞PPARγmRNA表达较单用脂多糖刺激组显著升高.这些结果提示HDL可能通过上调PPARγ的表达、抑制脂肪细胞分泌白细胞介素8分泌.3T3-L1 adipocytes were cultured with various concentrations of high-density lipoprotein （ HDL, 0, 10, 50, and 100 μg/ml ） for 16 h and with lipopolysaccharide （ LPS, 100 ng/ml ） for another 6 h. Interleukin-8 in the medium was determined by ELISA, and PPAR-γ mRNA expression by reverse transacription polymerase chain reaction （RT-PCR）. Interleukin-8 levels were increased in LPS-treated cells （ P〈0.05 ）, but decreased in HDL-treated cells in the dose-dependent manner. PPARγ mRNA expressions were increased in HDL-treated groups than those treated only with LPS. These results suggested HDL may decrease interleukin-8 secretion via up-regulating PPARγ expression in adipocytes.

关 键 词：高密度脂蛋白 脂肪细胞 白细胞介素8 PPARΓ 

分 类 号：R686[医药卫生—骨科学]