胰岛素经VEGF-A/VEGFR2途径促进恒河猴视网膜血管内皮细胞的血管新生  

Insulin promotes rhesus retinal vascular endothelial cells angiogenesis via VEGF-A/VEGFR2 pathway

作　　者：周赛君[1] 陈松[2] 于珮[1] 于德民[1] 

机构地区：[1]天津医科大学代谢病医院,卫生部激素与发育重点实验室,300070 [2]天津市眼科医院

出　　处：《中华内分泌代谢杂志》2010年第10期891-893,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金项目（30971393）;天津市科技支撑重点项目（08ZCGYSF01700）;天津市卫生局科技基金重点项目（09KZ01）

摘　　要：用人胰岛素处理恒河猴视网膜血管内皮细胞系RF/6A,测定其增殖、迁移、管腔形成情况和血管内皮生长因子A（VEGF-A）及其受体（VEGFR）的表达与磷酸化.与空白对照组相比,胰岛素促进RF/6A细胞增殖、迁移和管腔形成（均P＜0.01）、促进VEGF-A mRNA的表达和蛋白的活性（均P＜0.05）;促进VEGFR2 mRNA的表达和蛋白的磷酸化（均P＜0.01）,而对VEGFR1 mRNA的表达影响无统计学意义（P＞0.05）Rhesus retinal vascular endothelial cell line RF/6A cells were treated with human insulin. Cell proliferation, migration, and lumen formation, as well as the expressions of vascular endothelial growth factor-A （ VEGF-A ）, VEGF-A receptors, and phosphorylated receptors were measured. Insulin promoted RF/6A cell proliferation, migration, and lumen formation （ all P〈0. 01 ）. Insulin increased the expression of VEGF-A mRNA and improved its protein activity （ all P〈0. 05 ）, and promoted the expression of VEGFR2 mRNA and its phosphorylation （ both P〈0. 01 ）. There was no significant difference in the expression of VEGFR1 mRNA among the groups （ P〉0. 05 ）.

关 键 词：胰岛素 恒河猴视网膜血管内皮细胞系RF/6A 血管内皮生长因子 血管内皮生长因子受体 血管新生 

分 类 号：R686[医药卫生—骨科学]