活血止痛汤对硬膜外瘢痕中线粒体凋亡途径相关基因的影响  

Effects of Huoxuezhitong recipe on the pathway of mitochondrial apoptosis and the expression of related gene in epidural scar tissue

作　　者：范希玲[1] 周辉[2] 夏志敏[2] 牛海鑫[3] 罗均君[3] 

机构地区：[1]浙江大学医学院附属第一医院,浙江杭州310003 [2]杭州市中医院,浙江杭州310007 [3]浙江中医药大学

出　　处：《中国骨伤》2010年第10期776-779,共4页China Journal of Orthopaedics and Traumatology

基　　金：浙江省中医药管理局科技项目(编号:2008CB057)

摘　　要：目的:探讨中药活血止痛汤对硬膜外瘢痕中线粒体凋亡途径及Caspase3,9,Bax,Bcl2相关基因表达水平的影响。方法:雌性新西兰白兔60只(体重:2.5～3.0kg)按随机数目表分成假手术组(A)、空白对照组(B)、透明质酸钠组(C)和活血止痛汤组(D,主要药物有当归20g、赤芍20g、红花20g、乳香15g、没药15g等11味),每组15只。除A组外,其余各组手术切除L4,5椎板,造成1.0cm×1.0cm硬脊膜裸露区。C组于硬膜囊外均匀地涂上一层透明质酸钠约0.5ml,B、D组以等量生理盐水处理,术后2周内D组予活血止痛汤(2.5ml/kg,每日1次,连续14d)灌胃。术后第2、4、8周末每组各处死5只,逆转录酶链反应(RT-PCR)测定其硬膜外瘢痕组织中Caspase3,9,Bax,Bcl2的表达水平。结果:RT-PCR检测发现各组Caspase3,9,Bax,Bcl2的表达水平在各个时期均与A组存在统计学差异(P<0.01)。第2、4、8周时,C组和D组Caspase3,9,Bcl2的表达水平均明显低于B组(P<0.05),Bax则明显高于B组(P<0.05);在第8周时,D组Caspase3,9,Bax,Bcl2的表达水平与C组亦有统计学意义(P<0.05)。结论:活血止痛汤通过调节Caspase3,9,Bax,Bcl2的表达,可进一步诱导线粒体的凋亡通路,从而达到预防硬膜外瘢痕增生、粘连的效果。Objective：To study the effect of Huoxuezhitong recipe on the pathway of mitochondrial apoptosis and the ex-pression of related gene（Caspase 3, 9,Bax,Bcl 2） in epidural scar tissue. Methods：A total of 60 New Zealand rabbits,rang-ing in weight from 2.5 to 3.0 kg,were randomly divided into sham operative group （group A）,control group （group B）,sodium hyaluronate group（group C）,Huoxuezhitong recipe group（group D,included Danggui 20 g,Chishao 20 g,Honghua 20 g,Rux-iang 15 g,Myrrh 15 g etc.） with 15 rabbits in each group. Except for group A,L4,5 vertebral plate were resected leading to 1.0 cm ×1.0 cm dura mater exposed area,which were covered with 0.5 ml sodium hyaluronate in group C,covered with same amount of saline in group B and D. At 2 weeks after operation,the rabbits of group D were administered with Huoxuezhitong recipe （2.5 ml / kg,once a day,continuous 14 d）. Five rabbits of each group were selected randomly and were killed at 2,4 and 8 weeks after operation;and the expression of Caspase 3, 9,Bax,Bcl 2 in scar tissue were surveyed by semiquantitative re-verse transcription polymerase chain reaction （RT-PCR）. Results：The results by RT-PCR showed significant different in ex-pressions of Caspase 3, 9,Bax,Bcl 2 of group A and others groups at each period（P0.01）. At 2,4,8 weeks after operation, compared with group B,the expressions of Caspase 3, 9,Bcl 2 in group C and D were decreased （P0.05）,but the expression of Bax in group C and D were increased （P0.05）. At 8 weeks after operation,the expressions of Caspase 3, 9,Bax,Bcl 2 in group D was significant decreased compared with group C（P0.05）. Conclusion：Huoxuezhitong recipe can regulate the levels of expression of Caspase 3, 9,Bax,Bcl 2 mRNA and further induce the pathway of mitochondrial apoptosis,thereby,achieve the effect of prophylaxis for the proliferation and conglutination of epidural scar tissue

关 键 词：瘢痕 线粒体 基因 动物 实验 中草药 

分 类 号：R681.5[医药卫生—骨科学]