肾脂肪囊来源脂肪干细胞的体外培养及其生物学特性的研究  

Study on the biological characteristics of adipose stem cells derived from renal adipose capsule cultured in vitro

作　　者：吴畏[1] 郑红光[2] 张德伟[2] 梅煜明[2] 

机构地区：[1]辽宁医学院肾脏病科,辽宁锦州121011 [2]沈阳军区总医院肾病科,辽宁沈阳110015

出　　处：《细胞与分子免疫学杂志》2010年第11期1078-1081,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:研究肾脂肪囊内脂肪干细胞(ADSCs)的培养条件及生物学特性,为干细胞治疗肾脏疾病寻找更佳的细胞来源。方法:分别取大鼠肾脂肪囊及腹股沟区脂肪组织,分离培养ADSCs进行对比,细胞达80%融合时传代,观察细胞形态和增殖情况;采用MTT比色法测定细胞生长曲线;采用流式细胞术(FCM)检测细胞表面抗原的表达。结果:①相同质量肾脂肪囊和腹股沟区脂肪组织分离培养原代ADSCs显示,肾脂肪囊分离出ADSCs的数量显著超过腹股沟区脂肪组织;肾脂肪囊来源的ADSCs 5～6 d可达80%融合,而腹股沟区脂肪来源ADSCs则需要10～12 d。两者细胞均成梭形成纤维样细胞形态。②生长曲线分析显示肾脂肪囊ADSCs较腹股沟区ADSCs增殖迅速。③流式细胞术检测示两种干细胞的CD29抗原表达率均>99%,肾脂肪囊ADSCs CD44表达率为52.92%,腹股沟区ADSCs表达率为75.41%,内皮细胞标志抗原CD31均阴性。结论:与其他部位来源ADSCs相比,肾脂肪囊来源ADSCs具有分离量大,增殖迅速等特点,可在短时间内扩增到较大数量,可能成为治疗肾脏疾病的新的干细胞来源。AIM： To investigate the culture conditions and biological characteristics of the adipose-derived stem cells（ADSCs） isolated from renal adipose capsule so as to find a better source of stem cells for the treatment of kidney disease.METHODS： Renal adipose capsules and groin adipose tissues were taken from rats.The isolated and cultured ADSCs from the two regions were compared.When approximately 80% cell fusion were achieved,cell passage was established.To observe cell morphology and proliferation,we adopted MTT colorimetry to record cell growth curve,and utilized FCM to detect the expressions of cell surface antigen.RESULTS： ① The primary ADSCs showed that the number of ADSCs isolated from the renal adipose capsules significantly exceed that from the groin adipose tissues at the same quality.About 80% ADSCs derived from the renal adipose capsules fused in 5-6 days,while the ADSCs from the inguinal fat tissues needed 10-12 days approximately.Both were spindle-like or fibroblast-like in shape.② The analysis of the growth curve showed that the proliferation of ADSCs from renal fat capsule was faster than that from inguinal fat tissues.③ FCM showed that CD29 antigen expression rates of ADSCs from both parts exceeded 99%.The expression rates of the renal ADSCs for CD44 was 52.92%,while the expression rate of the groin ADSCs was 75.41%.The marking antigens of the endothelial cells for CD31 were negative.CONCLUSION： Compared with the ADSCs taken from the inguinal region,the renal ADSCs has the advantages of greater cell culture number and faster proliferation,which may become a new source of stem cells to treat the renal disease.

关 键 词：肾脂肪囊 脂肪干细胞 生物学特性 组织工程 

分 类 号：R392.11[医药卫生—免疫学]