日本血吸虫rSj26GST疫苗抑制小鼠肝脏虫卵肉芽肿形成的效果观察  

Effect observed on hepatic egg granuloma inhibitted in mice after using rsj26GST vaccine from Schistosoma japonicum

作　　者：杜健[1] 李晶[2] 房修罗[3] 景志敏[1] 

机构地区：[1]西北民族大学医学院,甘肃兰州730030 [2]甘肃省肿瘤医院骨与软组织肿瘤科,甘肃兰州730050 [3]桂林医学院附属医院感染科,广西桂林541001

出　　处：《细胞与分子免疫学杂志》2010年第11期1092-1094,共3页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:日本血吸虫rSj26GST疫苗抑制小鼠肝脏虫卵肉芽肿形成的效果观察。方法:将含有rSj26GST编码基因的菌株E.coliBL21/p ET28a涂布LB/Kana/IPTG/X-gal平板,培养、收集细菌、超声碎菌、分离、纯化,分别取5μL进行SDS-PAGE,观察纯化结果,采用BCA法测定重组蛋白的浓度。45只BALB/c小鼠随机分为2组:A组(感染对照组):于第0周每鼠经背部皮下多点注射50μL PBS及等体积福氏完全佐剂(CFA),第2、第4周每鼠皮下多点加强注射50μLPBS加等体积福氏不完全佐剂(IFA);B组(rSj26GST组),同A组,唯将PBS改为50μg(50μL)rSj26GST。上述两组分别于末次免疫2周后每鼠经腹部皮肤感染日本血吸虫尾蚴(30±2)条。C组(正常对照组):未作任何处理。攻击感染45 d剖杀小鼠,测量肝脏单个虫卵肉芽肿的大小,采用ELISA测定血清透明质酸及层黏连蛋白含量。结果:rSj26GST疫苗组小鼠虫卵肉芽肿的直径为(191.6±26.3)μm,显著小于感染对照组的(267.7±28.6)μm(P<0.05)。透明质酸和层黏连蛋白水平,rSj26GST疫苗组明显低于感染对照组(P<0.05),两者均高于正常对照组。结论:rSj26GST疫苗具有一定的抗虫卵肉芽肿及降低免疫病理损伤作用。AIM： To study the anti-schistosomiasis effect in mouse immunized with rSj26GST vaccine.METHODS： The E.coli BL21/pET28a of rSj26GST gene was coated LB/Kana/IPTG/X-gal pan,cultured,collecting bacteria,bacteria broken,separation,purification.5 μL purified protein were carried out from SDS-PAGE analysis,then to observe the results of purified protein.Each mouse in infection control group was boosted with 50 μL PBS on 0 weeks later,2 weeks later and 4 weeks later.While each mice rSj26GST vaccine group was boosted with rSj26GST vaccine.Two weeks after last immunization,each mouse was challenged with 30±2 cercariae of S.japonicum Chinese strain.The control group wasn＇t do some treatment.The mice were sacrificed at the 45th day post-infection.The size of single egg granulomas in livers was measured with micrometer.The level of hyaluronic acid（HA） and laminin（LN） in sera of the mice was determined by ELISA.RESULTS： The mean hepatic egg granuloma diameters of the rSj26GST vaccine group（191.6±26.3）μm was significantly smaller than that in infection control group（267.7±28.6） μm（P0.05）.The evel of HA and LN in sera of rSj26GST vaccine vaccinated mice was markedly lower than in infection control group（P0.05）.CONCLUSION： The results showed that rSj26GST vaccine may act as an effective inhibitor against formation of egg granuloma and reduce immunopathological damage caused by Schistosoma japonicum in the host.

关 键 词：日本血吸虫 rSj26GST疫苗 肝虫卵肉芽肿 肝纤维化 

分 类 号：R383.24[医药卫生—医学寄生虫学]