七龙天对实验性肺动脉高压大鼠ET-1、LTC4的影响  

Influence of "Qilongtian Decoction" on ET-1 and LTC4 in experimental pulmonary arterial hypertension rats

作　　者：付义[1] 陈冰 杨春艳[1] 魏丹霞[1] 焦丽杰[1] 余晓玲[1] 吴洪波[1] 张爱华[1] 张俊图[1] 

机构地区：[1]云南省昆明市中医医院肺病科,云南昆明650011 [2]云南白药集团股份有限公司,云南昆明650118

出　　处：《上海中医药杂志》2010年第11期66-68,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：云南省昆明市科技计划项目资助(昆科计字09S090203号)

摘　　要：目的观察七龙天对实验性肺动脉高压(PAH)大鼠内皮素1(ET-1)、白三烯C4(LTC4)的影响。方法雄性SD大鼠随机分为6组:空白对照组(A)、模型组(B)、硝苯吡啶组(C)、七龙天低剂量组(D)、七龙天中剂量组(E)、七龙天高剂量组(F)。空白对照组大鼠以等量0.9%NaCl溶液腹腔注射,其余各组均以野百合碱(MCT)腹腔注射复制PAH模型造模。制模后14天开始,各组分别定时灌胃给药,每日1次,连续给药8天至检查前12h停药。以改良右心导管术测定肺血流动力学参数,用放射免疫(RIA)法测定血浆ET-1,酶联免疫吸附法(ELISA)测定血清LTC4的变化。结果与A组比较,其余各组大鼠PASP、PADP、MPAP、ET-1、LTC4水平较空白对照组均有所升高,且B组升高最显著(P<0.01);与B组比较,C、D、E、F组均可降低大鼠PASP、PADP、MPAP、ET-1水平(P<0.05,P<0.01);与C、D组比较,E、F组大鼠PASP、PADP、MPAP、ET-1水平降低更明显(P<0.05);各用药组对LTC4的影响差异无统计学意义(P>0.05)。结论七龙天能够有效降低实验动物的肺动脉压,其机制与降低ET-1含量有关。Objective To study the effects of ＂Qilongtian Decoction＂ on plasma endothelin（ET-1） and leukotrienes C4（LTC4） in pulmonary arterial hypertension（PAH） rats. Methods The PAH model in male SD rats was induced by intraperitoneal injection of monocrotaline（MCT）. Nitro-pyridine was used as active control. Parameters of pulmonary hemodynamics were detected by improved right catheterization. Plasma endothelin（ET-1） was detected by radioimmunoassay（RIA）. The changes of leukotrienes C4（LTC4） was observed by enzyme-linked immunosorbent assay（ELISA）. Results ＂Qilongtian Decoction＂ and Nitro-pyridine could significantly reduce the levels of mean pulmonary arterial pressure （MPAP） and ET-1 in PAH rats（P0.05 or P0.01）, and little influence the level of LTC4（P0.05）; and ＂Qilongtian Decoction＂ in middle dose and high dose had better effects than Nitro-pyridine（P0.05）. Conclusion ＂Qilongtian Decoction＂ can effectively reduce MPAP in PAH rats by reducing ET-1 content.

关 键 词：肺动脉高压 七龙天 内皮素1 白三烯C4 药理学 

分 类 号：R285.5[医药卫生—中药学]