延髓头端腹内侧核群的GABA_A受体部分介导丙泊酚在中脑导水管周围灰质腹外侧区诱发的痛觉过敏作用  

Partial Mediation Effect of GABA_A Receptor in the Rostral Ventromedial Medulla on Propofol-Induced Hyperalgesia at Ventrolateral Portion of the Periaqueductal Gray of Rats

作　　者：王擒云[1] 杨建平[1] 冯昌栋[1] 戴体俊[2] 

机构地区：[1]苏州大学附属第一医院麻醉科,江苏苏州215006 [2]徐州医学院,江苏徐州221002

出　　处：《苏州大学学报（医学版）》2010年第5期907-909,914,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30872442);苏州大学附属第一医院青年创新项目(YQN02)

摘　　要：目的观察丙泊酚在中脑导水管周围灰质腹外侧区(vlPAG)诱发的痛觉过敏是否通过延髓头端腹内侧核群(RVM)的GABAA受体介导。方法采用热板法和福尔马林试验,将大鼠随机分组,丙泊酚和荷包牡丹碱分别采用vlPAG和RVM置管及微量注射给药。分别以舔后爪潜伏时间和疼痛评分为痛阈指标。结果两种疼痛模型中丙泊酚在vlPAG微量注射后(丙泊酚组)均引起痛觉过敏(P<0.01),预先在RVM注射荷苞牡丹碱(丙泊酚+荷包牡丹碱组)可部分拮抗这种作用。结论丙泊酚在vlPAG引起痛觉过敏,这种作用部分是通过RVM的GABAA受体中继而发挥作用的。Objective To observe whether the propofol-induced hyperalgesia at ventrolateral portion of the PAG（ vlPAG） is mediated by GABAA receptor at rostral ventromedial medulla （ RVM） of rats. Methods SD rats were randomly divided into four groups. The noxious response was evaluated by hot plate and formalin test. Bicuculline and propofol were separately microinjected into RVM and vlPAG. Results Hyperalgesia were induced in both hot-plate test and formalin test after propofol vlPAG microinjection. The hyperalgesia induced by propofol vlPAG microinjection was antagonized by bicuculline RVM microinjection （ P 0. 01） . Conclusion GABAA receptor in the rostral ventromedial medulla （ RVM） partially mediates propofol-induced hyperalgesia at ventrolateral portion of the PAG（ vlPAG） of rats.

关 键 词：丙泊酚 荷包牡丹碱 GABAA受体 热板法 福尔马林试验 延髓头端腹内侧核群 中脑导水管周围灰质 痛觉过敏 

分 类 号：R614.24[医药卫生—麻醉学] R971.2[医药卫生—外科学]