液态芯片检测多种肿瘤标志物在胃癌中的应用  

Clinical Significance of Detecting Multiple Tumor-Markers with Liquid Chip Technology in Gastric Cancer

作　　者：周凯[1] 李鹏 张萌 付晓君[1] 王旭义[1] 

机构地区：[1]安徽省蚌埠医学院第一附属医院普外科三病区,233004 [2]国家计划生育委员会科研所,北京市100000

出　　处：《中华全科医学》2010年第12期1534-1534,1547,共2页Chinese Journal of General Practice

摘　　要：目的①通过液态芯片(liquid Chip)技术检测胃癌(gastric cancer)患者和正常人群血清中多种肿瘤标志物(tum or markers)表达的差异;②探讨运用液态芯片技术进行血清肿瘤标志物联合检测对胃癌的诊断价值。方法应用多肿瘤标志物液态芯片检测系统,检测82例胃癌患者和82例正常对照者血清中4种常见肿瘤标志物。结果①胃癌组CEA、CA125、CA242、CYFRA211表达水平均显著高于正常对照组,差异有统计学差异意义(P<0.05);②胃癌组CEA、CA125、CA242、CYFRA211联合检测的阳性率是73.2%,而胃癌组单项检测CEA、CA125、CA242、CYFRA211的阳性率分别为41.5%、31.7%、37.8%、29.3%,均显著低于联合检测的阳性率(P<0.01)。结论血清CEA、CA125、CA242、CYFRA211是胃癌辅助诊断的理想指标;应用液态芯片技术联合检测肿瘤标志物CEA、CA125、CA242和CYFRA211有利于提高检出阳性率。Objective To explore the diagnosis value of combined detection of serum multi-tumor markers（TM） by liquid chip detective system in patients with gastric cancer. Methods Four kinds of tumor markers（CEA,CA125,CA242 and CYFRA211） in the serum of 82 patients with gastric cancer and 82 normal subjects were detected by the multi-tumor markers liquid chip diagnostic system. Results （1）The serum levels of CEA,CA125 ,CA242 and CYFRA211 in the patients with gastric cancer were significantly higher than that in control groups（P 〈 0.05 ） ;（2）The sensitivity of combined detection of CEA, CA125, CA242 and CYFRA211 in the patients with gastric cancer was 73.2% ,and was obviously higher than the positive rate（41.5% for CEA,31.7% for CA125, 37.8% for CA242 and 29.3% for CYFRA211, respectively） of single tumor marker detection （P 〈 0. 01 ）. Conclusion CEA, CA125,CA242 and CYFRA211 could be regarded as valuable tumor marker for gastric cancer. The combined detection of CEA, CA125, CA242 and CYFRA211 by the liquid chip technology can enhance the diagnostic sensitivity of gastric cancer by increase the positive rate.

关 键 词：液态芯片 胃肿瘤 肿瘤标志物 

分 类 号：R735.2[医药卫生—肿瘤] R730.45[医药卫生—临床医学]