HPLC法测定小鼠肝匀浆中乳梨醇的含量  

DETERMINATION OF LACTITOL IN MICE LIVER HOMOGENIZATIONS BY HPLC

作　　者：张敏波[1] 倪维芳[1] 黄宗玉[1] 

机构地区：[1]浙江省药品检验所

出　　处：《华西药学杂志》1999年第2期126-127,共2页West China Journal of Pharmaceutical Sciences

摘　　要：用高效液相色谱法建立了小鼠肝匀浆中乳梨醇的含量测定方法。乳梨醇的回收率为９９．９％，日内和日间ＲＳＤ均小于１．０％（ｎ＝５）。A HPLC method was developed for the determination of lactitol in mice liver homogenizations. The analysis was performed by using a Waters Sugar Pak1 column with redistilled water as mobile phase, the column temperature was maintained at 90℃, the differential refractometer was used. The average recovery was 99.9%. The limit of detection was 20 μg/ml (S/N=3). The linear range was 20￣500 μg/ml( r =0.9996). Within day and between day RSD were less than 1.0% ( n=5) . The result showed that lactitol liver was not found in mice.

关 键 词：小鼠 肝匀浆 乳梨醇 高效液相色谱 

分 类 号：R965[医药卫生—药理学] R927.2[医药卫生—药学]