实验性应激对链脲佐菌素诱导鼠血糖增高的机理研究  

Neuroendocrine mechanisms of impact by experimental stress on plasma glucose level in STZ KM mice

作　　者：姚树桥[1,2] 蔡伟雄[1,2] 戴晓阳[1,2] 龚耀先[1,2] 胡敏[1,2] 刘幼硕[1,2] 

机构地区：[1]湖南医科大学第二附属医院医学心理学中心 [2]湖南医科大学内分泌研究所

出　　处：《中华医学杂志》1999年第5期332-334,共3页National Medical Journal of China

基　　金：国家自然科学基金

摘　　要：目的探讨实验性应激影响链脲佐菌素诱导鼠（ＳＴＺ鼠）糖代谢改变的神经内分泌机理。方法将鼠分为Ａ组（ＳＴＺ鼠应激组，２０只）和Ｃ组（正常鼠应激组，１０只），给予６周旋转、限制、拥挤应激刺激；Ｂ组（ＳＴＺ鼠无应激组，２０只）和Ｄ组（正常鼠无应激组，１０只）不给予应激。实验前、后各测血糖１次，实验结束时收集血和下丘脑标本测定血浆胰岛素浓度、胰岛素敏感性指数（ＩＳＩ）、血浆去甲肾上腺素（ＮＥ）、肾上腺素（ＥＰＩ）和下丘脑β内啡肽（βＥＰ）含量。结果应激后Ａ组血糖（１０３±６２）ｍｍｏｌ／Ｌ，较其他３组显著增高，血浆胰岛素浓度显著下降，ＩＳＩ较Ｂ组显著降低（４６±０６）；应激后Ａ组ＮＥ、ＥＰＩ显著升高，分别为４３６５±４１０９、２７７６±３２４５ｎｇ／Ｌ，各实验组下丘脑βＥＰ含量组间差异无显著意义；实验后血糖与各神经内分泌指标之间有低至中度相关。结论实验性应激后ＳＴＺ鼠血糖显著增高很可能是其对应激刺激的肾上腺素能神经敏感性改变的结果，下丘脑βＥＰ可能参与这一过程的调节。Objective To explore the neuroendocrine mechanisms of effects by experimental stress on plasma glucose level in streptozotocin induced mice(STZ mice). Methods Forty STZ mice were divided randomly into Group A (STZ mice, stress) and Group B (STZ mice, no stress), and 20 normal KM mice in Group C (normal mice, stress) and Group D (normal mice, no stress). Group A and Group C were exposed to multiple stressors (restrain, rotation, crowding) for 6 weeks. Before and after the experiment, blood glucose(BG) levels were measured. By the end of experiment, blood and hypothalamus samples were collected to measure plasma insulin(I) concentrations, insulin sensitivity index(ISI), plasma norepinphine(NE), epinphin(EPI) and beta endorphin(EP) concentrations. ResultsAfter stress, the BG levels significantly increased and I concentrations decreased in Group A. Group A showed a greater decrease of ISI than that in Group B. There was significant difference in plasma NE and EPI concentrations among groups, and Group A showed marked increase. There was no significant difference in EP concentrations among groups. There were low to moderate correlations among these variables after stress. Conclusion The STZ mice likely have altered adrenergic sensitivity to stress, which might play a leading role in the neuroendocrine mechanism of hyperglycemia of STZ mice after stress. The EP might participate in this process of regulating glucose metabolism.

关 键 词：应激 链脲菌素 血糖 高血糖反应 

分 类 号：R587.302[医药卫生—内分泌]