井冈霉素高产菌株原生质体诱变筛选研究  

Research on Screening of Jinggangmycin strain with High-yield by Protoplast Mutation

作　　者：覃敬羽[1] 李世杰[1] 刘华梅 

机构地区：[1]湖北工业大学生物工程学院,湖北武汉430068 [2]武汉科诺生物科技有限公司

出　　处：《现代农业科技》2010年第22期38-39,共2页Modern Agricultural Science and Technology

摘　　要：以吸水链霉菌井冈变种k-121-5为出发菌株制备原生质体,并对其进行紫外诱变,通过筛选得到5株较为高产的菌株,经连续传代、摇瓶发酵,井冈霉素A产率比出发菌株提高14%～26%。treptomyces hygroscopicus var.jinggangensis Yen K-121-5 was accomplished protoplasts preparation.The protoplasts were treated with ultraviolet irradiation,screened on culture plate and obtained 5 high high-yield strains.Jinggangmycin A yields of the mutation strains was 14%～ 26% higher than the original strain by shaking flask fermentation after series transfer culturing.

关 键 词：井冈霉素 吸水链霉菌井冈变种 原生质体 诱变 筛选 

分 类 号：Q937[生物学—微生物学]