人骨膜间质干细胞移植后成骨能力及成骨起源的研究  

STUDY OF THE BONE FORMATION AND OSTEOGENESIS AFTER TRANSPLANTATION OF HUMAN PERIOSTEAL MESENCHYMAL STEM CELLS

作　　者：徐栋梁[1] 李佛保[1] 符气祯[1] 黄芝胜[1] 朱全胜[1] 张萌 李润梅 

机构地区：[1]中山医科大学附属第一医院骨科

出　　处：《中国修复重建外科杂志》1999年第3期133-136,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：广东省科委重点项目基金;广东省卫生厅科研基金

摘　　要：目的研究人骨膜间质干细胞异体移植后成骨能力及成骨起源。方法将幼儿骨膜分离培养后获得的骨膜间质干细胞悬液，移植于裸小鼠两侧背部皮下及老年股骨颈骨折处，观察并验证新骨形成。结果移植后第１１天肉眼可见皮下结节，第４周其直径为２～７ｍｍ（平均３．６ｍｍ）。组织学检查证实结节为结缔组织包裹的骨小梁球；人类特有的载脂蛋白Ｂ基因（ａｐｏＢ基因）的ＰＣＲ实验以及抗人类组织沉淀素反应均为阳性。注入无完整活细胞的骨膜间质干细胞冻融液及培养液的两组裸小鼠皮下均未见结节形成。从患者取出的新骨作组织学及ＰＣＲ检查显示，新骨ａｐｏＢ基因有两条电泳带，即含两个个体产物。结论裸小鼠皮下新骨结节含有人类细胞，移植的骨膜间质干细胞可直接成骨；异体移植细胞与宿主骨细胞可能共同成骨，为临床应用提供了重要的理论依据。Objective To study the bone formation and osteogenesis after transplantation of human periosteal mesenchymal stem cells(PMSC).Methods Suspension of PMSC which obtained from cell culture of periosteal segments in vitro were injected into the backs of nude mice subcutaneously ,and the fracture site of neck of femur in old person.Results Subdermal nodules were observed by naked eyes after 11 days of transplantation.4 weeks later ,their anatomic diameter reached 2～7 mm(averaged 3.6 mm).It was proved that the subdermal nodules were trabecular ball trapped with fibrous tissue.The nodules were investigated by human special apoB gene with PCR,and the test of anti human tissue precipitin reaction(AHTPR).The results of PCR and AHTPR were positive reaction.There were no subdermal nodules formed in the sites of injection of frozen melted PMSC or culture medium.The new callus in the sites of fracture were tested by PCR test,and two kinds of apoB gene products were detected.Conclusion The results indicated that the implanted PMSC could form new bone directly in nude mice ,and the cells of donor and recipient all could form new bone.

关 键 词：骨膜间质 干细胞移植 成骨发生 载脂蛋白B基因 

分 类 号：R683[医药卫生—骨科学] Q441[医药卫生—外科学]