采用激光微束将绿色荧光蛋白基因导入大肠杆菌  

Study on Introduction of the Green Fluorescent Protein Gene into E.coli by Using Laser Microbeam

作　　者：李泰明[1] 赵炎生[1] 王旻[1] 朱曦[1] 牛亚梅[1] 胡容[1] 

机构地区：[1]中国药科大学生物制药学院

出　　处：《中国药科大学学报》1999年第2期136-138,共3页Journal of China Pharmaceutical University

摘　　要：将ＮｄＹＡＧ三倍频脉冲激光微束系统用于含有绿色荧光蛋白基因的质粒ｐＳ６５Ｔ的转化，利用激光微束技术在经高渗液处理过的大肠杆菌ＤＨ５α上打孔，将质粒成功导入ＤＨ５α中，通过筛选、检测，证明该方法是一种新的，快速有效的转化方法。A laser microbeam system was introduced to the study of green fluorescent protein (GFP) gene transfer. Laser microbeam was used to break up cell wall of E.coli (DH5α). The plasmid pS65T of having GFP was successfuly introduced into DH5α and expressed.The strain was genetically stable. This method is proved to be quick and efficent in introducing,expressing,screeing and detection.

关 键 词：激光微束 绿色荧光蛋白 大肠杆菌 基因导入 

分 类 号：Q785[生物学—分子生物学]