甲磺酸帕珠沙星荧光光谱特性研究及应用  

Fluorescence Spectra of Pazufloxacin Mesylate and Its Appliation

作　　者：王琪[1] 庄严[1] 叶小会[1] 

机构地区：[1]江苏技术师范学院化学与环境工程学院

出　　处：《光谱实验室》2010年第6期2197-2201,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家科技支撑计划重大项目(2008BAC46B04);江苏技术师范学院自然基金项目(KYY08018)

摘　　要：研究了低浓度甲磺酸帕珠沙星水溶液的最佳激发波长,荧光光谱和同步共振光谱,不同实验条件对其荧光强度的影响。结果显示,甲磺酸帕珠沙星溶液在407nm出现明显的荧光峰,在375nm出现共振荧光峰,最佳激发波长为285nm。随浓度增加,407nm处的荧光强度线性增强,当浓度增至6×10^(-4)mol/L后,发生荧光猝灭,荧光强度线性减弱。不同的表面活性剂对甲磺酸帕珠沙星水溶液荧光强度有增强或猝灭作用。在pH为9.18硼砂的缓冲溶液中,甲磺酸帕珠沙星水溶液荧光强度最强。利用其荧光光谱特性,测定样品中甲磺酸帕珠沙星,结果满意。The optimal excitation wavelength of low concentration pazufloxacin mesylate aqueous solution,its fluorescence spectra and synchroresonance spetra,different experiment conditions which effect its fluorescence intensity have been studied. The results show that the pazufloxacin mesylate solution exhibits an obvious fluorescence peak centered at 406nm,a formant spectra at 375nm, for which the best excitation wavelength is 285nm. With the concentration increasing, the fluorescence intensity at 285nm increases linearly, and when the concentration was up to 6 × 10.4 mol/L, concentration quenching occurs,so the intensity linearly weakens. Various kinds of surfadants have enhanced or quenched pazufloxacin mcsylate solution＇s fluorescence intensity. Pazufloxacin mesYlate aqueous solution displays the strongest fluorescence intensity in borax buffer solution of pH 9.18. It has been applied to the direct determination of pazufloxacin mesylate in the samples with satisfactory results.

关 键 词：甲磺酸帕珠沙星 荧光光谱 荧光强度 

分 类 号：O657.32[理学—分析化学]