量子点CdSe/CdS-微囊藻毒素-LR抗体生物共轭体测定环境水样中的微囊藻毒素-LR  

Determination of Microcystin-LR in Environmental Water Samples by Bioconjugates Between CdSe/CdS Quantum Dots and Microcystin-LR Antibody

作　　者：孟元华[1] 周晓盼[1] 陶冠红[1] 

机构地区：[1]苏州大学材料与化学化工学部

出　　处：《光谱实验室》2010年第6期2354-2358,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(20345006;20575043)

摘　　要：建立了一种基于CdSe/CdS量子点(QDs)标记技术测定微囊藻毒素-LR(MC-LR)的方法。MC-LR抗体经1-乙基-3(3-二甲基胺基丙基)碳二亚胺盐酸盐(EDC)活化,与经巯基乙酸修饰的CdSe/CdS量子点进行共价连接,获得的QDs生物共轭体在待测物MC-LR的作用下发生荧光猝灭现象,荧光猝灭程度与MC-LR的浓度呈线性关系。该方法操作简单、检测速度快、检出限低达6.9×10^(-11)mol/L。A new method for the determination of microcystinqeucine-arginine （MC-LR） in water samples was developed based on the fluorescence quenching of bioconjugates between CdSe/CdS quantum dots （QDs） and MC-LR antibody in the presence of the analyte. After activated by 1-ethyl-3- （3-dimethyllaminopropyl） carbodiimidc hydrochloride,monoclonal anti-MC-LR antibody was covalently conjugated to the modified QDs by 2-mercaptoacetic acid to form their bioconjugates. The fluorescence intensity of the bioconjugates between QDs and monocional anti-MC-I.R antibody was quenched in the presence of MC-LR. A linear relationship between the percentage of fluorescence quenching and MC-LR concentrations was observed. This method is easy, fast with limit of detection of 6.9×10 -11mol · L-1（3σ）.

关 键 词：量子点 微囊藻毒素 荧光光谱 

分 类 号：O657.32[理学—分析化学]