三氟乙酸酐衍生化GC/MS/MS方法检验尿中吗啡  

Determination of Morphine in Urine by GC/MS/MS with Trifluoroacetic Anhydride Derivatization

作　　者：李国平[1] 

机构地区：[1]辽宁警官高等专科学校公安技术系

出　　处：《光谱实验室》2010年第6期2373-2377,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：辽宁省教育厅科研基金资助项目(20060423)

摘　　要：建立了人体尿中吗啡和O^6-单乙酰吗啡的衍生化GC/MS/MS定性、定量分析方法。以SKF_(525a)为内标,三氟乙酸酐为衍生化试剂,采用DB5-MS色谱柱对衍生化产物进行分析。尿中吗啡和O^6-单乙酰吗啡的质量浓度在0.05—10μg/mL范围内线性良好,线性相关系数r均为0.9901;方法回收率为65%—86%。检出限均为0.05ng/mL。该法操作简单,灵敏度高,适合作为法医毒物分析的常规方法。A methods for qualitative and quantitative analysis of morphine and OG-monoacetylmorphine in human urine were developed by GC/MS/MS with derivatization. SKF525a, was used as the internal standard,and trifluoroacetic anhydride （TFA） was used derivative reagent, separately, and the derivatized products were determinated by DBS-MS column. The good linearity was obtained within the mass concentration range of 0. 05--10ug/mL （r= 0. 9901）. The level of recovery was 65%-86%. The LOD of morphine and O6-monoacetylmorphine were all 0.05 ng/mL. The method is simply, highly sensitive, and can be used for forensic toxicological conventional analysis.

关 键 词：吗啡 O6-单乙酰吗啡 气相色谱/质谱/质谱法 三氟乙酸酐 衍生化 尿 

分 类 号：O657.63[理学—分析化学]